Entrecruzamiento, marcaje y modificación de proteínas

Thermo Scientific™ 2-mercaptoetanol Pierce™

Agente reductor del tiol puro, líquido (14 M), de beta-mercaptoetanol (bME, 2BME, 2-ME, b-mer), n.º de CAS 60-24-2; para la disociación de los enlaces de los disulfuros de proteínas (cistinas cisteínas). 2-MERCAPTOETHANOL 10ML

Thermo Scientific™ Pierce™ DTT (ditiotreitol)

Reduce disulfides in biological buffers and sample loading buffers with pure, crystalline dithiothreitol (DTT), the most popular protein disulfide reducing agent. DTT 5GM

Thermo Scientific™ Guanidina·HCl Pierce™

Pure, crystalline guanidium hydrochloride (GdnHCl, GuHCl), CAS 50-01-1; denaturant for protein biology methods (elution, solubilization, refolding, storage). GUANIDINE-HCL 500GM

Thermo Scientific™ Entrecruzador Pierce™ BS3 (Sulfo-DSS)

Crosslink amines to amines with BS3; composed of sulfo-NHS-ester groups separated by an 11.4A, water-soluble spacer arm that is useful for cell-surface protein crosslinking. BS3 1G

Thermo Scientific™ N-Etilmaleimida (NEM) Pierce™

Small, sulfhydryl-reactive (maleimide) compound to permanently block reduced cysteines of proteins or peptides to prevent disulfide bond formation. NEM OR N-ETHYLMALEIMIDE 25GM

Thermo Scientific™ Solución de guanidina·HCl Pierce™ de 8 M

Ready-to-use, 8M solution of guanidinium hydrochloride (GdnCl, GuHCl), CAS 50-01-1; dilute and pH-adjust to any concentration for protein biology methods. 8M GUANIDINE-HCL, 200 MLambient temp

Invitrogen™ Image-iT™ Green Hypoxia Reagent

Image-iT Green Hypoxia Reagent is a novel, fixable fluorogenic compound for measuring hypoxia in live cells. Image-iT Green Hypoxia Reagent, 1 vial

Thermo Scientific™ Gel reductor de disulfuro de TCEP inmovilizado Pierce™

Efficiently reduce protein or peptide disulfide bonds with this stable, odorless, beaded agarose resin on which Tris (2-carboxyethyl) phosphine is immobilized. 5 ML IMMOBILISED TCEP DISULFIDE REDUCING GEL 5MLStore in fridge at 4C

Thermo Scientific™ Biotina de sulfo-NHS-LC y kits de etiquetado EZ-Link™

Biotinylate protein amines (e.g., lysines) with this very popular mid-length (22.4A), water-soluble, NHS-ester biotinylation reagent for antibody or cell-surface labeling. EZ-LINK MICRO SULFO-NHS-LC-BIOTIN

Molecular Probes™ Dithiothreitol (DTT)

Dithiothreitol (DTT) DITHIOTHREITOL (DTT)

Thermo Scientific™ Colorantes de fluoresceína y FITC y kits de etiquetado Pierce™

Activated derivatives of green-fluorescent fluorescein dye to label antibodies, proteins and other molecules for fluorescence imaging (ex 494; em 518). FLUORESCEIN ANTIBODY LABELING KIT

Invitrogen™ DTT, 1M

DTT, 1M DTT, 1M 1 ML

Thermo Scientific™ Pierce™ TRITC Rhodamine Dyes and Labeling Kits

Label antibodies, proteins and other molecules for fluorescence imaging (ex 546; em 572-615) using these activated derivatives of red-fluorescent rhodamine dye. 25 MG NHS-RHODAMINE 25MG STORE IN FREEZER AT -20C

Molecular Probes™ BODIPY™ 493/503 NHS Ester (Succinimidyl Ester)

BODIPY™ 493/503 NHS Ester (Succinimidyl Ester) 4,4-DIFLUORO-1,3,5,7-TETR

Molecular Probes™ Click-iT™ HPG Alexa Fluor™ 488 Protein Synthesis Assay Kit

Click-iT™ HPG Alexa Fluor™ 488 Protein Synthesis Assay Kit CLICK-IT HPG ALEXA FLUOR 488

Molecular Probes™ 2',7'-Difluorofluorescein (Oregon Green™ 488)

2',7'-difluorofluorescein (Oregon Green™ 488) 2',7'-DIFLUOROFLUORESCEIN

Thermo Scientific™ Reactivo de Traut Pierce™'

2-Iminothiolane-HCl (CAS 4781-83-3); an amine-reactive thiolation reagent to cap protein primary amines with sulfhydryl groups for immediate crosslinking. 500 MG 2-IMINOTHIOLANE HYDROCHLORIDE 500MG STOREin Fridge at 4C

Molecular Probes™ Qdot™ 800 ITK™ Carboxyl Quantum Dots

Qdot™ 800 ITK™ Carboxyl Quantum Dots QDOT 800 ITK CARBOXYL QUANTUM

Thermo Scientific™ Pierce™ AEDP Crosslinker

Crosslink amines to carboxyls with this spacer containing an internal disulfide bond; use EDC to modify functional groups on proteins and surfaces. AEDP 50MG

Merck™ Calbiochem™ EDTA, Disodium Salt, Dihydrate, Molecular Biology Grade

Application in Molecular Biology 1KG EDTA, Disodium Salt, Dihydrate, Molecular Biology Grade

Molecular Probes™ Texas Red™ Sulfonyl Chloride, mixed isomers

Texas Red™ Sulfonyl Chloride, mixed isomers TEXAS RED(R) SULFONYL CHL

Molecular Probes™ 6-TAMRA, SE (6-Carboxytetramethylrhodamine, Succinimidyl Ester), single isomer

6-TAMRA, SE (6-Carboxytetramethylrhodamine, Succinimidyl Ester), single isomer 6-CARBOXYTETRAMETHYLRHODA

Thermo Scientific™ Biotina de sulfo-NHS-SS y kits de etiquetado EZ-Link™

Biotinylate protein amines with this cleavable, mid-length (24.3A), water-soluble, NHS-ester biotinylation reagent containing a cleavable disulfide bond in the spacer arm. EZ-LINK MICRO SULFO-NHS-SS-BIOTIN

Thermo Scientific™ Kit de biotinilización de anticuerpos Pierce™ para IP

Marque anticuerpos primarios con biotina para su uso en experimentos de inmunoprecipitación con gránulos magnéticos o de agarosa mediante el uso del kit de biotinilación de anticuerpos Thermo Scientific™ Pierce™ para IP. 8RXN Pierce Antibody Biotinylation Kit for IP, 8 reactions

Molecular Probes™ 6-FAM (6-Carboxyfluorescein), single isomer

6-FAM (6-Carboxyfluorescein), single isomer 6-CARBOXYFLUORESCEIN (6-F

Thermo Scientific™ Proteinasa K Pierce™

Cleave at the carboxyl-side of aliphatic, aromatic or hydrophobic residues and digest-inactivate DNase and RNase in nucleic acid purification using this protease. PROTEINASE K 100MG

Thermo Scientific™ Entrecruzador SMCC Pierce™

Crosslink amines to sulfhydryls with this mid-length crosslinker composed of NHS-ester and maleimide groups at ends of a stabilizing cyclohexane-containing, 8-atom spacer arm. SMCC, 50 MGne-1-carboxylate 50mg Store in Fridge at 4C

Thermo Scientific™ Entrecruzador DTSSP Pierce™

Crosslink amines to amines with Sulfo-DSP; composed of sulfo-NHS-ester groups and disulfide spacer arm (12.0A) that is cleavable with reducing agents. DTSSP 50MG

Thermo Scientific™ Hidroxilamida·HCl Pierce™

NH2OH (CAS 7803-49-8) to cleave Asn-Gly peptide bonds or deprotect sulfhydryls following S-acetylthioacetate modification with SATA reagent. HYDROXYLAMINE-HCL 25GM

Molecular Probes™ Alexa Fluor™ 488 NHS Ester (Succinimidyl Ester)

Alexa Fluor™ 488 NHS Ester (Succinimidyl Ester) ALEXA FLUOR(R) 488 CARBOX

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