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IBA Lifesciences StarGate pPSG-IBA Acceptor Vectors for E. coli Expression
Logra una expresión de alto nivel en E. coli por parte del promotor T7 al proporcionar un entorno genético específico para una expresión y purificación óptimas de proteínas no tóxicas.
220.00€
Especificaciones
| Formato | Líquido |
|---|---|
| Para utilizar con (equipo) | Sistema Stargate™, E. coli BL21(DE3) |
| Agente inductor | Huésped de E. coli con el gen de la polimerasa de ARN T7 (p. ej BL21(DE3) ) |
| pH | 8 |
| Promotor | T7 |
| Código de producto | Marca | Vector | Precio | Cantidad y disponibilidad | |||||
|---|---|---|---|---|---|---|---|---|---|
| Código de producto | Marca | Vector | Precio | Cantidad y disponibilidad | |||||
|
15633758
|
IBA Lifesciences
5-4200-001 |
Longitud total: 3469 | N/A | ||||||
|
15643758
|
IBA Lifesciences
5-4203-001 |
Longitud total: 3469 | N/A | ||||||
|
15653758
|
IBA Lifesciences
5-4205-001 |
Longitud total: 3469 | N/A | ||||||
|
15663758
|
IBA Lifesciences
5-4223-001 |
Longitud total: 3469 | N/A | ||||||
|
15673758
|
IBA Lifesciences
5-4225-001 |
Longitud total: 3469 | N/A | ||||||
|
15683758
|
IBA Lifesciences
5-4233-001 |
Longitud total: 3469 | N/A | ||||||
|
15693758
|
IBA Lifesciences
5-4235-001 |
Longitud total: 3469 | N/A | ||||||
|
15603768
|
IBA Lifesciences
5-4243-001 |
Longitud total: 3469 | N/A | ||||||
|
15613768
|
IBA Lifesciences
5-4245-001 |
Longitud total: 3469 | N/A | ||||||
|
15623768
|
IBA Lifesciences
5-4262-001 |
Longitud total: 3469 | N/A | ||||||
|
15633768
|
IBA Lifesciences
5-4263-001 |
Longitud total: 3469 | N/A | ||||||
|
15643768
|
IBA Lifesciences
5-4264-001 |
Longitud total: 3469 | N/A | ||||||
|
15653768
|
IBA Lifesciences
5-4265-001 |
Longitud total: 3469 | N/A | ||||||
|
15663768
|
IBA Lifesciences
5-4303-001 |
Longitud total: 3469 | N/A | ||||||
|
15673768
|
IBA Lifesciences
5-4305-001 |
Longitud total: 3469 | N/A | ||||||
|
15683768
|
IBA Lifesciences
5-4323-001 |
Longitud total: 3469 | N/A | ||||||
|
15693768
|
IBA Lifesciences
5-4343-001 |
Longitud total: 3469 | N/A | ||||||
|
15603778
|
IBA Lifesciences
5-4345-001 |
Longitud total: 3469 | N/A | ||||||
|
15613778
|
IBA Lifesciences
5-4362-001 |
Longitud total: 3469 | N/A | ||||||
|
15623778
|
IBA Lifesciences
5-4364-001 |
Longitud total: 3469 | N/A | ||||||
|
15633778
|
IBA Lifesciences
5-4367-001 |
Longitud total: 3469 | N/A | ||||||
|
15643778
|
IBA Lifesciences
5-4368-001 |
Longitud total: 3469 | N/A | ||||||
Description
pPSG-IGA E. coli expression vectors:
pPSG-IBA vectors use the T7 promoter and T7 RNA polymerase for high-level transcription of the gene of interest.
- High-level expression in E. coli by bacteriophage T7 promoter
- High-level transcription by T7 RNA polymerase in BL21 strains
- High-level expression of non-toxic proteins
- Induction by IPTG
- Suitable for in vitro transcription/translation
StarGate System
IBA developed StarGate to provide a simple, fast and highly efficient cloning process for genes of interest (GOI) for use in functional expression, purification, detection, immobilization or separation of recombinant proteins:
- Minimal extra modification of the gene due to short combinatorial sites
- Inherent high-level cloning efficiency due to a directed reaction (no equilibrium)
- GOIs are cloned to obtain a Donor Vector; 2 or more genes can be combined using Fusion Cloning
- Genes are then transferred into Acceptor Vectors that provide optimal genetic surroundings
- The final expression vector, the Destination Vector, is then placed into the respective host
Specifications
| Líquido | |
| Huésped de E. coli con el gen de la polimerasa de ARN T7 (p. ej BL21(DE3) ) | |
| T7 | |
| de 2 °C a 8 °C para uso frecuente, –20 °C para almacenamiento a largo plazo | |
| 5 μg | |
| Clonación de genes de interés para su uso en la expresión funcional, purificación, detección, inmovilización o separación de proteínas recombinantes |
| Sistema Stargate™, E. coli BL21(DE3) | |
| 8 | |
| Tampón TE de 20 μl: 10 mm de Tris-HCl, 1 mm de EDTA/5 μg | |
| 250 ng/μl | |
| Transparente |
Safety and Handling
missing translation for 'shelfLife' : 12 meses
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Product Title
IBA Lifesciences StarGate pPSG-IBA Acceptor Vectors for E. coli Expression