Thermo Scientific™ SureQuant™ IP-MS Sample Preparation Kits

Descripción

Los kits de preparación de muestras Thermo Scientific SureQuant IP-MS (proteína A/G o estreptavidina) proporcionan una solución optimizada para la inmunoprecipitación y la preparación de péptidos de MS de muestras antes del análisis de LC-MS/MS. Estos kits se han verificado rigurosamente utilizando numerosos antígenos objetivo multiplexados con diferentes niveles de expresión. La familia de productos SureQuant permite el uso de kits de preparación de muestras de inmunoprecipitación (IP) y módulos de cuantificación de péptidos juntos o de forma independiente para el análisis SRM/PRM.

• Complete—kits include reagents for successful immunoprecipitation and MS peptide preparation for target proteins for SRM/PRM analysis
• Multiplex—IP-MS kits have been designed for multiplex IP using both Protein A/G and Streptavidin magnetic beads
• Flexible—universal reagents enable individualized selection of targets for IP to be used alone or combined with SureQuant peptide quantitation panels
• Optimized—simplified and condensed procedure to prepare samples for LC-MS analysis
• Low background—binding, wash, and elution buffers optimized to remove contaminants and minimize enrichment of background proteins

The SureQuant Protein A/G and Streptavidin IP-MS Sample Preparation kits have been designed to support targeted quantitation using SureQuant or SRM/PRM analysis. The kits contain high-quality Pierce Protein A/G or Streptavidin magnetic beads together with reagents that have been optimized for MS compatibility. The streamlined procedure enables digestion immediately after the immunoprecipitation elution step, facilitating MS sample preparation in ∼4 hours for same-day LC-MS/MS analysis.

Applications:
• Enrichment of low abundance targets to improve MS sensitivity
• Identification of target proteins that are not detectable by western blotting
• Quantitation of target proteins by LC-MS analysis
• Screening of antibodies for IP-MS
• Enrichment of target proteins upstream of targeted analysis (SRM)