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Invitrogen™ TOPO™ TA Cloning™ Kit for Sequencing, with One Shot™ MAX Efficiency™ DH5α-T1R E. coli

Provides a highly efficient, 5 minute, one-step cloning strategy (“TOPO Cloning”) for the direct insertion of Taq polymerase-amplified PCR products into a plasmid vector for sequencing

Marca:  Invitrogen™ K459501

Detalles adicionales : Peso : 0.04000kg

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Código de producto. 10104512

  • 888.00€ / cada uno


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Descripción

Descripción

TOPO TA Cloning kits
• Alta eficiencia
• 'One-step'
Especificaciones

Especificaciones

DH5α
Box 1:
• Topoisomerase I-activated pCR™4-TOPO® vector
• PCR buffer
• Salt solution
• dNTPs
• Control template
• T3, T7, M13 forward, and M13 reverse primers
• Control PCR primers
• Sterile water

Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.

Box 2:
• One Shot® Chemically Competent or Electrocomp™ E. coli
• S.O.C. medium
• Supercoiled pUC19 control plasmid

Store at -68 to -85°C.
TOPO-TA Cloning Vectors
TOPO®-TA
One Shot™
25 reactions
Videos
Documentos

For Research Use Only. Not for use in diagnostic procedures.