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Cdk1/Cdc2 (pY15) Mouse, Unlabeled, Clone: 44, BD
Mouse Monoclonal Antibody
Marca: BD Biosciences 612307
Descripción
Progression of the mammalian cell cycle is regulated by phosphorylation of many key proteins. Several classes of cyclins (A-E) act as regulatory subunits for cyclin-dependent kinases (cdks). Cdk1/Cdc2 (p34 [cdc2]) is the catalytic subunit of the maturation promoting factor (MPF), which includes the regulatory subunit cyclin B. During late S and G2 phase, cyclin B synthesis increases, allowing it to bind Cdc2. This begins the transition into M-phase of the mammalian cell cycle by initiating a series of phosphorylation and dephosphorylation events that lead to activation of the Cdc2/cyclin B complex. After binding to cyclin B, cdc2 is phosphorylated on Thr-14, by Myt1, and Tyr-15, by wee1 or mik1, yielding an inactive pre-MPF complex during G2 phase. Phosphorylation of cdc2 on Thr-161 is performed by a cdk7/cyclin H complex and is necessary for activation of the cdc2 complex. Dephosphorylation of Thr-14 and Tyr-15 by CDC25 occurs at the end of G2 phase and completes activation of the cdc2/Cyclin B complex and facilitates entry into mitosis. During mitosis, cyclin B is targeted for degradation and Cdc2 becomes inactive again.
Immunofluorescence, Western Blotting

Especificaciones
Cdk1/Cdc2 (pY15) | |
Monoclonal | |
250μg/mL | |
p34 [cdc2] | |
Mouse | |
Affinity Purified | |
RUO | |
Primary | |
Store undiluted at -20°C. |
Western Blot | |
44 | |
Unconjugated | |
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide. | |
Phosphorylated Human Cdc2 (pY15) Peptide | |
150 μg | |
Cell Biology | |
Human, Mouse, Rat | |
IgG1 |
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For Research Use Only.