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  7. Invitrogen™ SuperScript™ IV Single Cell/Low Input cDNA PreAmp Kit
  8. 17787433

Invitrogen™ SuperScript™ IV Single Cell/Low Input cDNA PreAmp Kit

Designed for efficient cDNA synthesis and amplification directly from intact single cells (1 to 1,000) or low amounts of total RNA (2 pg to 10 ng). Kit contains all required components to perform cell lysis, reverse transcription (RT), and PCR amplification in a convenient premixed format.

Marca:  Invitrogen™ 11752480

4267.90 EUR hasta el 2023-03-27
Use el código promocional "20585" para aplicar la oferta.


Detalles adicionales : Peso : 0.32700kg

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Código de producto. 17787433

  • 6370.00€ / 480 reacciones
Fecha estimada de envío: 26-04-2023
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Descripción

Descripción

  • Designed for efficient cDNA synthesis and amplification directly from intact single cells (1 to 1,000) or low amounts of total RNA (2 pg to 10 ng)
  • Kit contains all required components to perform cell lysis, reverse transcription (RT), and PCR amplification in a convenient premixed format
  • A combination of superior enzymes (SuperScript IV Reverse Transcriptase and Platinum SuperFi U DNA Polymerase) enables high yields, sensitivity, and accuracy
  • Due to the short reaction times and minimal hands-on time, this simple one-tube preamplification protocol can be completed in just over two hours
  • The high quality global cDNA obtained can be used for next-generation sequencing (NGS) applications or for gene expression analysis by real-time PCR
  • Superior sensitivity for easy detection of low abundant targets from as low as single cell or 2 pg of total RNA
  • High quality cDNA for full-length transcript information with uniform coverage
  • Global preamplification compatible with downstream analysis by NGS or real-time PCR

Each pack contains:

  • 10X Lysis Buffer, 48 μL
  • RNase Inhibitor, 19 μL
  • Capturing Oligonucleotide, 48 μL
  • 4X SuperScript IV cDNA Synthesis Master Mix, 240 μL
  • Template Switching Oligonucleotide, 48 μL
  • 2X Platinum SuperFi U Preamplification Master Mix, 2 x 1.2 mL
  • Preamplification primer, 48 μL
  • Water, nuclease-free, 2 x 1.25 mL
Especificaciones

Especificaciones

Ten packs, each containing:
• 10X Lysis Buffer (48 μL)
• RNase Inhibitor (19 μL)
• Capturing Oligonucleotide (48 μL)
• 4X SuperScript IV cDNA Synthesis Master Mix (240 μL)
• Template Switching Oligonucleotide (48 μL)
• 2X Platinum SuperFi U Preamplification Master Mix (2 x 1.2 mL)
• Preamplification primer (48 μL)
• Nuclease-free water (2 x 1.25 mL)

Store at –25°C to –15°C.
High
30 min.
SuperScript IV
Real Time PCR (qPCR)
Reverse Transcription
Cells or RNA
Kit
Platinum SuperFi U DNA Polymerase
50°C
Dry Ice
480 Reactions
Up to 10 kb
Videos
SDS
Documentos

Documentos

Certificados
Ofertas especiales

Ofertas especiales

For Research Use Only. Not for use in diagnostic procedures.