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PRKCN (PKD3) Recombinant Human Protein
PRKCN (PKD3) Recombinant Human Protein
Marca: Invitrogen PV3692
Detalles adicionales : Peso : 0.00945kg
Descripción
The PKC family of serine⁄threonine kinases, including PRKCN (PKD3), is activated intracellularly by signal transduction pathways. In humans, at least 12 different PKC polypeptides have been identified. These isoforms differ in primary structure, tissue distribution, subcellular localization, mode of action in vitro, response to extracellular signals, and substrate specificity. PKC alpha, beta I, beta II and gamma form the conventional family; their activities are Ca2+- and phospholipid-dependent.Especificaciones
| NP_005804 | |
| Pharma and Biopharma, Protein Assays and Analysis, Target and Lead Identification and Validation, Kinase Assay | |
| 127.9kDa | |
| 10μg | |
| For research use only. Not for use in diagnostic procedures | |
| PRKCN (PKD3), PKC nu | |
| PRKCN was pre-diluted in enzyme dilution buffer and assayed in 50mM Tris (pH 7.5), 10mM MgCl2, 0.25mM EGTA, 0.1mM Na3VO4, 5mM β-glycerophosphate, 2.5mM DTT, 0.01% Triton™ X-100, 200μM ATP, 33μM MAPKAPK2 kinase peptide substrate (KKLNRTLSVA) and trace [32P]-γ-ATP for 10 minutes at 30°C | |
| PRKCN (PKD3) Recombinant Human Protein | |
| Human | |
| Baculovirus | |
| Serine/threonine kinase |
| 0.39mg/mL | |
| 23683, 9139 | |
| PRKCN (PKD3) Recombinant Human Protein | |
| –80°C | |
| 1 Each | |
| PRKCN (PKD3) Recombinant Human Protein | |
| PKN | |
| 240nmoles of phosphate transferred to MAPKAPK2 kinase peptide substrate (KKLNRTLSVA) per minute per mg of total protein at 30°C. Activity determined at a final protein concentration of 0.83μg/mL | |
| GST Tag | |
| Kinase and inhibitors | |
| 95% by SDS-PAGE |
For Research Use Only. Not for use in diagnostic procedures.