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Thermo Scientific™ Soluciones de sustrato NBT/BCIP Pierce™

Powders and ready-made nitro-blue tetrazolium and 5-bromo-4-chloro-3'-indolyphosphate solutions for chromogenic detection of alkaline phosphatase probes.

Marca:  Thermo Scientific™ 34042

Detalles adicionales : Peso : 1.81440kg

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Código de producto. 10434175

  • 200.00€ / 250 ml


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Descripción

Descripción

The combination of NBT (nitro-blue tetrazolium chloride) and BCIP (5-bromo-4-chloro-3'-indolyphosphate p-toluidine salt) yields an intense, insoluble black-purple precipitate when reacted with alkaline phosphatase, a popular enzyme conjugate for antibody probes. Pierce NBT and BCIP powders are available individually and in two convenient, ready-to-use solutions (with and without a levamisole suppressor of endogenous phosphatase activity).

Highlights:

  • AP substrate – for detection of alkaline phosphatase (alk-phos) activity on solid media, including nitrocellulose and PVDF membranes and fixed tissue samples

  • Chromogenic – no special equipment needed for visualization; produces purple-black precipitate that is easy to photograph

  • Package options – choose individual NBT and BCIP powders, or preformulated, ready-to-use solutions with or without levamisole suppressor

1-Step™ NBT/BCIP

  • Ideal for immunoblotting applications
  • Minimal assay-to-assay variability

1-Step NBT/BCIP Plus Suppressor

  • Ideal for staining tissues assayed with calf intestinal alkaline phosphatase without the interference caused by endogenous phosphatases
  • Contains 1mM levamisole to inhibit endogenous phosphatase activity
Especificación

Especificación

Detección de fosfatasa alcalina
Cromogénico
Único componente
25 usos a 10 ml cada uno
Solución de sustrato 1-Step NBT/BCIP Pierce
Solución exclusiva de nitroazul de tetrazolio (NBT) y 5 bromo-4-cloro-3-indolil fosfato (BCIP)
250 ml
4 °C
Videos
Documentación