missing translation for 'onlineSavingsMsg'
Learn More
Learn More
NIH/3T3 (mouse NIH/Swiss embryo) Nuclear extract lysate, Denatured; Abnova
Marca: Abnova L018V3.50ug
Este artículo no se puede devolver.
Vea la política de devoluciones
Descripción
Inmunotransferencia (Western Blotting)
Especificaciones
| Western Blot | |
| Nuclear extract cell lysate (denatured) | |
| Nuclear extract was prepared by using a modified protocol of Dignam et al. Cells were Harvested and homogenized in Buffer A, and then centrifugated at 25,000 g for 20 minutes to remove cytoplasm and pellet the nuclei. The pellet was re-suspended in Buffer C, and then the suspensions were centrifuged to collect nuclear extract. The supernatant was dialyzed against Buffer D. The dialysate was then centrifuged, divided into aliquots, and stored at -80°C. The protein concentration was determined by the method of Bradford (Bio-Rad protein assay, microplate standard assay). The lysate was adjusted to 2.5 mg/ml, and then mixed with 5X Sample Buffer to become final 2 mg/ml in 1X Sample Buffer. The lysate was heated at 95°C for 5 min, and cooled rapidly. | |
| 50 μg | |
| Mouse |
| Embryo, Contact-inhibited | |
| Buffer A: 10mM HEPES pH 7.9, 1.5mM MgCl2, 10mM KCl, 0.5 mM DTT. Buffer C: 20mM HEPES pH 7.9, 25%(v/v) Glycerol , 0.42M NaCl , 1.5mM MgCl2, 0.2 mM EDTA, 0.5 mM DTT & 0.5 mM PMSF. Buffer D : 20mM HEPES pH 7.9, 20%(v/v) glycerol, 50mM KCl, 0.2 mM EDTA, 0.5 mM DTT & 0.5 mM PMSF. | |
| 12.5% SDS-PAGE Stained with Coomassie Blue. | |
| In ready-to-use 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue). | |
| Almacenar a -80°C. Dividir para evitar repetir la congelación y descongelación. |
Corrección del contenido de un producto
Proporcione sus comentarios sobre el contenido del producto rellenando el siguiente formulario.
Título del producto
NIH/3T3 (mouse NIH/Swiss embryo) Nuclear extract lysate, Denatured; Abnova > 50 μg
¿Detecta una oportunidad de mejora?Comparta una corrección de contenido