Invitrogen™ Kit ELISA para lisados celulares de MIP-3 alfa/CCL20 humana

Descripción

The Human MIP-3 alpha/CCL20 ELISA quantitates Hu MIP-3α in human cell or tissue lysates. The assay will exclusively recognize both natural and recombinant Hu MIP-3α. Principle of the method The Human MIP-3α solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

La proteína-3 inflamatoria macrófaga alfa (MIP-3 alfa), también denominada CCL20, se expresa en el hígado, los pulmones, los ganglios linfáticos y los linfocitos de la sangre periférica. MIP-3 alfa está fuertemente regulada al alza por señales inflamatorias, y regulada a la baja por la citoquina antiinflamatoria IL-10. MIP-3 alfa envía señales a través del receptor acoplado a la proteína G, CCR6, y actúa como un quimioatrayente para los linfocitos y las células dendríticas.