Thermo Scientific™ MabCaptureC Affinity Matrix

Descripción

MabCaptureC Affinity Matrix is an agarose-based, high-binding-capacity Protein A chromatography resin with a rProtein A ligand produced in yeast. The engineered rProtein A binds at the CH2-CH3 interface of the Fc-region of IgGs and shows cross binding to the VH3 domain of IgGs.

MabCaptureC™ Affinity Matrix is suitable for the purification of monoclonal antibodies.The rProtein A ligand is immobilized on a highly cross-linked and uniform-sized agarose-based bead with an average particle size of 75 μm. The uniform particle size delivers superior performance characteristics compared to traditional Protein A resins.

Features of the MabCaptureC™ Affinity Matrix include:

• High dynamic binding capacity for mAbs of >50 g/L at 4.8 min residence time
• Purification of mAbs and a subpopulation of scFv's and Fab fragments containing VH3 binding domains
• Clean and sanitize with standard sodium hydroxide agents for chromatography products
• Excellent scalability
• Non-animal derived

Main characteristics

• Matrix: agarose-based, epoxide activated
• Average particle size: 75 ± 10 μm
• Ligand: recombinant C domain of Protein A
• Ligand coupling method: epoxide coupling
• Binding capacity: >50 g IgG/L resin at 4.8 min residence time and 10% breakthrough
• Elution conditions: 0.1 M glycine pH 3.0, or 20–100 mM sodium citrate or acetate, pH 3.0
• Cleaning conditions: 0.2 M sodium hydroxide for 15 minutes every cycle
• Operating pressure: ≤3 bar
• Pressure flow: >500 cm/h at 3 bar in a 2.6 x 20 cm column
• Formulation buffer: 20% (v/v) ethanol

For process development and resin screening, MabCaptureC™ is also available in the following formats:

• MabCaptureC™ MiniChrom Column, 1 mL (Cat. No. 5943662001)
• MabCaptureC™ MiniChrom Column for process development, 5 mL (5943662005)
• MabCaptureC™ RoboColumn, 200 μL (5943662200)
• MabCaptureC™ RoboColumn, 600 μL (5943662600)