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His-Tag Mouse anti-Tag, Clone: 1B7G5, Proteintech

Mouse Monoclonal Antibody

Marca:  Proteintech 66005-1-IG-150UL

Código de producto. 16857495

  • 189.00€ / 150 microlitros

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Descripción

Descripción

The His-tag antibody is a useful tool for monitoring His-tagged proteins, and recognizes His-tags placed at N-terminal, C-terminal, and internal regions of fusion proteins expressed in bacteria, insect, and mammalian cells.

The 6x His tag is a synthetic oligo peptide consisting of 6 consecutive histidine residues (HHHHHH). The His tag is commonly expressed as a tag at either N-or C-terminal regions of recombit proteins to allow isolation or purification by immobilized metal affinity chromatography. Epitope tagging is a technique in which a known epitope is fused to a recombit protein using genetic engineering. By choosing a particular epitope and recombit protein combination, epitope tagging makes it possible to detect proteins for which no antibody is available. His epitope tagged proteins that contain a stretch of histidine residues at the carboxyl or amino terminus are used for purification of recombit proteins by means of metal chelate chromatography due to metal binding properties of histidine clusters on His tagged proteins. For example, the affinity of the histidine-tag motif to Ni2+ by chelation is strong and selective enough to enable purification of the protein to homogeneity by affinity chromatography on a Ni2+-NTA adsorbent.
Especificaciones

Especificaciones

His-Tag
Monoclonal
1.34 mg/mL
PBS with 50% glycerol and 0.02% sodium azide; pH 7.3
Mouse
Protein G
RUO
-20°C
Liquid
Tag
Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot
1B7G5
Unconjugated
6x His, 6x His, His Tag, 6histag, 6XHis, HHHHHH, HIS, His probe, His Tag, His-probe, HISTIDINE TAGGED
Peptide
150 μL
Primary
Antibody
IgG1
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Título del producto
His-Tag Mouse anti-Tag, Clone: 1B7G5, Proteintech > 150 μL; Unconjugated

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