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Invitrogen™ GSK-3A Human Protein
Easily screen for kinase binding. Thermo Fisher Invitrogen™ GSK-3A Human Protein recombinant fusion protein consists of full-length glycogen synthase kinase 3 alpha (GSK3A) and a glutathione S-transferase (GST) tag. It is validated in the LanthaScreen™ Eu Kinase Binding Assay.
Marca: Invitrogen™ PR9188A
Detalles adicionales : Peso : 0.01000kg
Descripción
The LanthaScreen™ Eu Kinase Binding Assay is a TR-FRET assay that lets you measure inhibitor binding to non-activated kinases, perform simple off-rate measurements and makes simple the study of type III (allosteric) inhibitors.
The LanthatScreen™ Eu Kinase Binding Assay is easy to optimize and run. The assay uses a simple mix-and-read format with a single one-hour incubation and no development steps. Simultaneous binding of an Alexa Fluor™ conjugate or tracer” to a tagged kinase is detected by addition of a europium-labeled anti-tag antibody, in this case anti-GST. Simultaneous binding of the tracer and antibody to the kinase results in a high degree of fluorescence resonance energy transfer (FRET), while displacement of the tracer with a kinase inhibitor results in a loss of FRET.
- Compatible and validated with the LanthaScreen™ Eu Kinase Binding Assay
- Expressed in insect cells
- GST tagged for use with Eu-labeled GST antibody
- No special measures were taken to activate this kinase
GSK3A for the LanthaScreen Kinase Binding Assay
Glycogen synthase kinase 3 alpha (GSK3A) is a multifunctional protein serine/threonine kinase implicated in the control of several regulatory proteins and transcription factors. (GSK3A Alias: DKFZp686D0638)
This insect cell-expressed recombinant fusion protein of full-length GSK3A and GST tag has been validated in the LanthaScreen™ Eu Kinase Binding Assay with the Tracer 236 and Eu-labeled GST reagents.
Especificaciones
NP_063937.2 | |
50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.5mM EDTA, 0.02% NP-40, 2mM DTT and 50% glycerol. | |
100μg | |
RUO - research use only | |
GSK-3A | |
Human | |
GST Tag | |
Frozen |
Enzyme and Protein Activity Assay, Kinase Assay, Protein Assays and Analysis, Protein Biology | |
78.4kDa | |
Store at -80°C. At first use, aliquot and store at -80°C to avoid multiple freeze-thaws. | |
Full Length | |
GSK Kinase Family | |
Recombinant | |
Baculovirus |