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Invitrogen™ GSK-3A Human Protein
Easily screen for kinase binding. Thermo Fisher Invitrogen™ GSK-3A Human Protein recombinant fusion protein consists of full-length glycogen synthase kinase 3 alpha (GSK3A) and a glutathione S-transferase (GST) tag. It is validated in the LanthaScreen™ Eu Kinase Binding Assay.
1826.00€ - 7890.00€
Especificaciones
| Número de acceso | NP_063937.2 |
|---|---|
| Para utilizar con (aplicación) | Enzyme and Protein Activity Assay, Kinase Assay, Protein Assays and Analysis, Protein Biology |
| Formulación | 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.5mM EDTA, 0.02% NP-40, 2mM DTT and 50% glycerol. |
| Peso molecular | 78.4kDa |
| Requisitos de almacenamiento | Store at -80°C. At first use, aliquot and store at -80°C to avoid multiple freeze-thaws. |
| Código de producto | Marca | Cantidad | Precio | Cantidad y disponibilidad | |||||
|---|---|---|---|---|---|---|---|---|---|
| Código de producto | Marca | Cantidad | Precio | Cantidad y disponibilidad | |||||
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15428723
|
Invitrogen™
PR9188A |
100μg |
1826.00€
100 microgramos |
Fecha estimada de envío: 07-06-2024 Inicie sesión para ver el stock |
Por favor, inicie sesión para comprar este producto. ¿No tiene usuario web? Regístrese hoy mismo. | ||||
|
15428813
|
Invitrogen™
PV6127 |
1mg |
7890.00€
1 miligramo |
Fecha estimada de envío: 17-05-2024 Inicie sesión para ver el stock |
Por favor, inicie sesión para comprar este producto. ¿No tiene usuario web? Regístrese hoy mismo. | ||||
Descripción
The LanthaScreen™ Eu Kinase Binding Assay is a TR-FRET assay that lets you measure inhibitor binding to non-activated kinases, perform simple off-rate measurements and makes simple the study of type III (allosteric) inhibitors.
The LanthatScreen™ Eu Kinase Binding Assay is easy to optimize and run. The assay uses a simple mix-and-read format with a single one-hour incubation and no development steps. Simultaneous binding of an Alexa Fluor™ conjugate or tracer” to a tagged kinase is detected by addition of a europium-labeled anti-tag antibody, in this case anti-GST. Simultaneous binding of the tracer and antibody to the kinase results in a high degree of fluorescence resonance energy transfer (FRET), while displacement of the tracer with a kinase inhibitor results in a loss of FRET.
- Compatible and validated with the LanthaScreen™ Eu Kinase Binding Assay
- Expressed in insect cells
- GST tagged for use with Eu-labeled GST antibody
- No special measures were taken to activate this kinase
GSK3A for the LanthaScreen Kinase Binding Assay
Glycogen synthase kinase 3 alpha (GSK3A) is a multifunctional protein serine/threonine kinase implicated in the control of several regulatory proteins and transcription factors. (GSK3A Alias: DKFZp686D0638)
This insect cell-expressed recombinant fusion protein of full-length GSK3A and GST tag has been validated in the LanthaScreen™ Eu Kinase Binding Assay with the Tracer 236 and Eu-labeled GST reagents.
Especificaciones
| NP_063937.2 | |
| 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.5mM EDTA, 0.02% NP-40, 2mM DTT and 50% glycerol. | |
| Store at -80°C. At first use, aliquot and store at -80°C to avoid multiple freeze-thaws. | |
| Full Length | |
| GSK Kinase Family | |
| Recombinant | |
| Baculovirus |
| Enzyme and Protein Activity Assay, Kinase Assay, Protein Assays and Analysis, Protein Biology | |
| 78.4kDa | |
| RUO - research use only | |
| GSK-3A | |
| Human | |
| GST Tag | |
| Frozen |