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Applied Biosystems™ Eukaryotic 18S rRNA Endogenous Control (FAM™/MGB probe, non-primer limited)
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Eukaryotic 18S rRNA Endogenous Control (FAM™/MGB probe, non-primer limited)

350.00€ - 1208.00€

Especificaciones

Número de acceso X03205.1.
Tamaño del amplicón 187
Contenido y almacenamiento Conjuntos de sondas de cebador de control
Método de detección Sonda de cebado
Formato Tubo
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Código de producto Marca N.º de reacciones Precio Cantidad y disponibilidad  
10370545
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Applied Biosystems™
4333760T
125 reacciones
350.00€
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10015904
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Applied Biosystems™
4333760F
500 reacciones
724.00€
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10618295
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Applied Biosystems™
4352930E
2500 reacciones
1208.00€
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Descripción

Descripción

The Applied Biosystems™ Eukaryotic 18S rRNA Endogenous Control (FAM™ MGB Probe, Non-Primer Limited) is intended as an endogenous control. It allows relative gene expression quantification in cDNA samples when used with other gene expression assays. Probe is labeled with 6FAM™ dye - MGB and the primers are not limited. Can be used for singleplex PCR reactions. Endogenous control is to be used with Inventoried and Non-Inventoried TaqMan™ Gene Expression Assays, Custom TaqMan™ Gene Expression Assays, and⁄or Custom TaqMan™ Primers and Probes.

Assay Details:

Gene Symbol: 18S
RefSeq: X03205.1
Probe Exon Location: NA
Amplicon Size: 187
Corresponding TaqMan Assay ID: Hs99999901_s1

TaqMan™ Endogenous Controls

Eliminate months of assay design, formulation, and testing by using TaqMan™ Endogenous Controls as your controls to quantify gene expression. This convenient collection of pre-designed probe and primer sets enables you to normalize the amount of sample RNA or DNA in a reaction.

Complete Solution for Quantitative Gene Expression

Having a hard time deciding what controls to use to quantify gene expression—even with detailed information on biological systems? Now, with TaqMan™ Endogenous Controls, you can avoid all the trial-and-error of selecting controls for most common human, mouse, rat, and eukaryotic genes.

Simple to Use

All components of the TaqMan™ Endogenous Controls are QC tested, formulated into a single 20X mix, and functionally tested. The controls are not only simple to use, but they are also fully compatible with universal conditions for two-step RT-PCR. Just add TaqMan™ Universal PCR Master Mix (with or without AmpErase™ UNG) and your cDNA sample to generate sensitive, reproducible, and truly quantitative gene expression data on Applied Biosystems instruments including the Applied Biosystems 7900HT, 7300, 7500 Real-Time PCR Systems, and the 7000 and 7700 Sequence Detection Systems.

Flexible Offering

We build each endogenous control using our proven 5' nuclease chemistry. For maximum flexibility, you can choose between two different reporter dyes and two quenchers:
  • A FAM™ dye-labeled TaqMan™ MGB probe (250nM, final concentration) and two unlabeled PCR primers (900nM each)
  • A VIC™ dye-labeled TaqMan™ MGB probe (250nM, final concentration) and two unlabeled PCR primers (150nM each y primer limited)
  • A VIC™ dye-labeled TAMRA™ probe (250nM, final concentration) and two unlabeled PCR primers (150nM each y primer limited)

    Choosing the Right Endogenous Control

    Endogenous controls can normalize the expression levels of target genes by correcting differences in the amount of cDNA that is loaded into PCR reaction wells. For best results, verify that the endogenous control is consistently expressed in the sample set to be tested. Endogenous control expression must be uniform across all samples in the study. For multiplexing, ensure that the gene expression level of the endogenous control is greater than that of the target.

    Multiplex vs. Singleplex PCR

    All TaqMan™ Endogenous Controls that contain probes labeled with the VIC™ reporter dye are primer limited. This allows multiplexing of TaqMan™ Endogenous Controls with target gene expression assays, provided that the control gene is more abundantly expressed than the target gene. All TaqMan™ Endogenous Controls that contain probes labeled with the FAM™ reporter dye are not primer limited and are not intended for multiplexing .

    For Research Use Only. Not for use in diagnostic procedures.
  • Especificaciones

    Especificaciones

    X03205.1.
    Conjuntos de sondas de cebador de control
    Tubo
    18S.
    FAM
    Temperatura ambiente
    ARNr 18S
    20X
    187
    Sonda de cebado
    RT-qPCR de 2 paso
    Embalaje sostenible
    FAM™, TaqMan™
    Eucariota, rata, ratón, humano
    Expresión génica
    Videos
    SDS
    Documentos

    Documentos

    Product Certifications

    For Research Use Only. Not for use in diagnostic procedures.