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Applied Biosystems™ Custom TaqMan™ SNP Genotyping Assay, non-human
Applied Biosystems TaqMan SNP Genotyping Assays use TaqMan 5´‐nuclease chemistry to amplify and detect specific polymorphisms in purified genomic DNA samples.
Marca: Applied Biosystems™ 4332075
Detalles adicionales : Peso : 0.03000kg
Includes: Dos cabadores de PCR sin etiquetar: directo e inverso (cebadores a 900 nM de concentración final); 1 colorante VIC: La sonda con etiqueta MGB detecta la secuencia del alelo 1 (sondas a una concentración final de 200 Nm); 1 colorante 6FAM: la sonda con etiqueta MGB detecta la secuencia del alelo 2 (sondas a concentración final de 200 nM)
Descripción
Applied Biosystems TaqMan SNP Genotyping Assays use TaqMan 5´‑nuclease chemistry to amplify and detect specific polymorphisms in purified genomic DNA samples. Each assay enables genotyping of individuals for a single nucleotide polymorphism (SNP) and consists of two sequence-specific primers and two TaqMan minor groove binder (MGB) probes with non-fluorescent quenchers (NFQ). One probe is labeled with VIC dye to detect the Allele 1 sequence; the second probe is labeled with FAM dye to detect the Allele 2 sequence.
Custom TaqMan SNP Genotyping Assays can be easily designed at no extra charge by submitting target sequences confidentially to our secure Custom Assay Design Tool. The tool can generate assay designs targeting any SNP in any organism, offering maximum flexibility to meet your research needs.
Benefits:
• Proven—gold-standard TaqMan chemistry and robust assay designs deliver accurate, reproducible, and reliable results
• Easy—convenient single-tube format and simple workflow provide an easy path to trusted results; no optimization required
• Flexible—obtain assay designs for any SNP in any organism using our secure Custom Assay Design Tool, at no extra charge
• Tested—all custom assays are quality-control tested for synthesis accuracy and formulation completeness
Approximate ship time
4–6 days in North America and 6–10 days in Europe
The free Custom Assay Design Tool can generate assays for the detection of SNPs, MNPs, and indels of up to six bases. These custom assays are designed, synthesized, formulated, optimized, and quality control tested.
TaqMan SNP Genotyping Assays require only three reaction components for PCR: purified genomic DNA (1–20 ng), the assay solution, and TaqMan Genotyping Master Mix (or another compatible master mix) (sold separately).
All assay designs are the product of our industry-leading bioinformatics pipeline, optimized over the course of more than a decade by leveraging manufacturing and assay performance data. TaqMan Assays have been cited in over 40,000 publications and are backed by more than 350 patents.
Recommended master mix (sold separately): TaqMan Genotyping Master Mix (Cat. No. 4371355)
Especificaciones
| 1 tubo que contiene una mezcla de 40x (tamaños S y M) u 80x (tamaño L) de ensayo preformulado (2 sondas y 2 cebadores). Almacenar a entre -15 y -25 °C. |
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| Embalaje sostenible | |
| Ensayos TaqMan personalizados | |
| 40X | |
| TaqMan™ |
| 5000 (placa de 384 pocillos); 1000 (placa de 96 pocillos) | |
| Temperatura ambiente | |
| No humano | |
| Sistema 7500, sistema 7500 Fast, sistema 7900HT, StepOne™, StepOnePlus™,™ Sistema VIIa 7, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, sistema* para PCR QuantStudio™ 12k Flex ProFlex, VeritiPro*, SimpliAmp*, MiniAmp*, termociclador* automatizado * Si se utiliza un termociclador para la amplificación de PCR, la lectura previa opcional y la lectura posterior deben realizarse por separado en un sistema para PCR en tiempo real para detectar y registrar señales fluorescentes. |
For Research Use Only. Not for use in diagnostic procedures.