Thermo Scientific™ CaptureSelect™ C-tagXL Affinity Matrix

Descripción

CaptureSelect C-tagXL Affinity Matrix combines a unique selectivity for a small 4-amino acid peptide tag (E-P-E-A, glutamic acid-proline-glutamic acid-alanine) with the benefits of a robust and high quality affinity matrix. It purifies C-terminal tagged proteins with high affinity and selectivity, even in the presence of urea and guanidine HCl, from complex mixtures like cell culture harvests and periplasmatic fractions in a one-step process. Mild elution conditions at neutral pH can be applied using magnesium chloride or propylene glycol, which ensures high activity recoveries of pH-sensitive target proteins. C-tagXL Affinity Matrix recognizes the E-P-E-A tag sequence when fused either directly to the C-terminus of a protein or through a linker between the C-terminus and the E-P-E-A tag.

Features of C-tagXL Affinity Matrix include:

• Mild elution, making it suitable for pH-sensitive proteins
• Binding of the tagged proteins under denaturing conditions (like dissolved inclusion bodies)
• Excellent scalability
• Non-animal-derived

CaptureSelect C-tagXL Affinity Matrix purifies C-terminal tagged E-P-E-A proteins directly from complex source materials in a single step with high purity and yield.

Free of animal components

CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).

Main characteristics:

• Matrix: agarose-based, epoxide-activated
• Average particle size: 65 ± 10 μm
• Ligand: CaptureSelect C-tagXL affinity ligand
• Ligand coupling method: epoxide coupling of the ligand
• Binding capacity: 400 nmol/mL resin depending on flow rate, column height, and contact time
• Elution conditions: Acidic: 20 mM citric acid or acetic acid, pH 3–4, or 0.1 M glycine pH 3.0; Neutral: 20 mM Tris, 2.0 M MgCl₂ pH 7; 20 mM Tris, 1.0 M NaCl, 50% (v/v) propylene glycol; 20 mM Tris 2 mM S-E-P-E-A.
• Flow characteristics: 50–200 cm/h (up to 2 bar)
• Formulation buffer: 20%(v/v) ethanol