missing translation for 'onlineSavingsMsg'
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Invitrogen™ Human ULK1, GST Tag Recombinant Protein
ULK1 is a serine/threonine protein kinase that plays critical role during initial stages of autophagy which is a vital response to nutrient starvation. Recombinant human ULK1 (1-649) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Marca: Invitrogen™ PV6430
Detalles adicionales : Peso : 0.01000kg
Descripción
The conserved C-terminal domain (CTD) of ULK1 controls the regulatory function and localization of the protein. Knockdown of ULK1 inhibits the autophagic response as well as inhibiting rapamycin-induced autophagy consistent with a role downstream of mTOR. ULK1 forms a complex with FIP200 and ATG13 and this complex is essential for starvation-induced autophagy. Both FIP200 and ATG13 are critical for correct localization of ULK1 to the pre-autophagosome and stability of ULK1 protein. ULK1 is phosphorylated by the mTOR pathway in a nutrient starvation-regulated manner.
- Validated in LanthaScreen™ Kinase Binding Assay
- Protocols available for simple use and optimized performance
- Multiple pack sizes available for convenient use
Especificaciones
| Kinase Assay | |
| 10μg | |
| aa 1-649 | |
| Recombinant | |
| Storage Buffer: 50 mM Tris-HCl pH 7.5, 150 mM NaCl, 0.25 mM DTT, 10 mM glutathione, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol Store at -68 to -85°C. Aliquot at first use to avoid multiple freeze-thaws. |
|
| Serine/Threonine Kinase | |
| Baculovirus | |
| Recombinant, Fragment | |
| Serine/Threonine Kinases |
| 96.1 kDa | |
| ATG1, UNC51, Unc51.1, FLJ38455 | |
| Human | |
| GST Tag | |
| Biochemical binding/kinetic | |
| ULK1 | |
| Kinases & Inhibitors | |
| Liquid |