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Invitrogen™ RAGE (STK30) Mouse ELISA Kit
Sandwich ELISA Kit
Marca: Invitrogen™ EMSTK30X5
Detalles adicionales : Peso : 0.33800kg
Includes: Placas precubiertas, estándar, anticuerpo detector, conjugado HRP, diluyentes, tampón de lavado, cromógeno, solución de parada y cubiertas de placa.
Descripción
The Mouse RAGE (STK30) ELISA quantitates Ms RAGE in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms RAGE. Principle of the method The Mouse RAGE solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Capaz de fosforilar varios sustratos exógenos y de someterse a autofosforilación.Especificaciones
| Q9WVS4 | |
| 15 pg/ml | |
| ELISA tipo sándwich de fase sólida | |
| Kit de ELISA | |
| Plasma, Sobrenadante, Suero | |
| Ensayos de 5 x 96 | |
| ELISA, ensayos de proteínas, biología de proteínas, biología del cáncer, inmunología, neurociencia, neurología, detección de proteínas, análisis de proteínas | |
| 26448 | |
| <12% | |
| Placas de 96 pocillos previamente recubiertos, patrón, concentrado de diluyente de muestra, anticuerpo de detección biotinilado, SAV-HRP, tampón de lavado, cromógeno, solución de parada, cubiertas de placa adhesivas | |
| 5 × 96 pruebas | |
| Otra investigación | |
| De 2 °C a 8 °C | |
| Ratón | |
| 4 h 45 min |
| De 13,72 pg/ml a 10 000 pg/ml | |
| De 15 pg/ml a 10,000 pg/ml | |
| Biotina | |
| Quinasa e inhibidores | |
| Reactividad cruzada: Este kit de ELISA no muestra reactividad cruzada con las siguientes citocinas analizadas: CD30L de ratón, CD30, CD40, CRG-2, CTACK, CXCL16, eotaxina, eotaxina-2, ligando Fas, fractalquina, GCSF, GM-CSF, IFN-gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alfa, IL- | |
| Ratón | |
| Lector de microplacas colorimétrico | |
| Mok | |
| <10% | |
| Peroxidasa de rábano picante (HRP) | |
| RUO | |
| 50 μl de plasma; 50 μl de suero; 100 μl de sobrenadante | |
| RAGE1, Rage, Stk30 | |
| 1 h 20 min |