Invitrogen™ Prostasin/PRSS8 Human ELISA Kit

Descripción

The Human Prostasin/PRSS8 ELISA quantitates Hu Prostasin in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Prostasin. Principle of the method The Human Prostasin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Este gen codifica un tripsinógeno, que es un miembro de la familia de tripsinas de las proteasas de serina. Esta enzima está altamente expresada en el epitelio prostático y es una de las varias enzimas proteolíticas que se encuentran en el líquido seminal. La proproteína se disocia para producir una cadena ligera y una cadena pesada que se asocian con un enlace disulfuro. Es activo en los enlaces peptídicos que involucran el grupo carboxilo de lisina o arginina.