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Molecular Probes™ CyQUANT™ Direct Cell Proliferation Assay

CyQUANT™ Direct Cell Proliferation Assay

Marca:  Molecular Probes™ C35011

Detalles adicionales : Peso : 0.00450kg

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Código de producto. 10422185

  • 325.00€ / 1 set


Stock EU 18
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Descripción

Descripción

Experience a more convenient workflow for your high-throughput cytotoxicity and proliferation screening projects with the CyQUANT™ Direct Cell Proliferation Assay–no washes, cell lysis, temperature equilibrations, or radioactivity required. DNA-based assays have been shown to be among the most sensitive cell health indicators and data concordance with metabolically based assays is excellent. The CyQUANT™ Direct assay is a highly sensitive and robust method to assess cell growth, viability, or compound toxicity in a range of applications, from high throughput screening to bioproduction.

Features of the CyQUANT™ Direct Cell Proliferation Assay include:

  • More convenient workflow for high-throughput screening–no washes, cell lysis, temperature equilibrations, or radioactivity required
  • Rapid protocol, high sensitivity and dynamic range
  • Measures independent of metabolic state of cells on standard fluorescent plate readers
  • Multiplex assays using a spectrally distinct fluorescent or a luminescent readout

    Rapid and convenient, with a large dynamic range
    The CyQUANT™ Direct assay is a fluorescence-based proliferation and cytotoxicity assay for microplate readers with a linear detection range from less than 100 to 20,000 cells per well in most cell types. The no-wash, homogenous format and fast add-mix-read protocol makes the CyQUANT™ Direct assay ideal for HTS applications. The assay can be completed in one hour, with no washes, no cell lysis, or temperature equilibrations required. The signal is stable for several hours, affording additional work flow convenience.

    How it works
    The CyQUANT™ Direct assay is based on a cell-permeant DNA-binding dye in combination with a masking dye reagent. Cellular DNA is highly regulated and estimates of cell numbers based on DNA are very accurate. The masking dye blocks staining of dead cells or cells with compromised cell membranes resulting in only healthy cells being stained. The CyQUANT™ Direct assay, therefore, measures proliferation as well as membrane integrity, another measure of cell health. Users of metabolically based cell health assays can readily compare historical data with results from the CyQUANT™ Direct assay, as concordance assays based on metabolism or energy (ATP) has been shown to be excellent. Furthermore, it is possible to multiplex with spectrally distinct fluorescent probes or luminescence assay kits because cells are not lysed. Furthermore, the fluorescent signal allows the user to switch between HTS and HCS plate readers if multiplex assays require different platforms.
  • Especificaciones

    Especificaciones

    1 Kit(s)
    2 to 25°C
    For HTS reader, microplate reader, microscope, spectrophotometer
    For cell viability, proliferation and function
    1536-well plate, 384-well plate, 96-well plate
    CyQUANT™ Cell Proliferation Assay Kit, 10 Microplates, Green, 1536, 384 and 96-well Plate Format, Fluorescent Detection Method, CyQUANT™ Direct Label/Dye, 2 to 25°C Storage, 508/527nm Excitation/Emission Wavelength, Use with Microplate Reader
    Fluorescent
    Contains 0.5mL CyQUANT™ Direct nucleic acid stain (component A), 2.5mL CyQUANT™ Direct background suppressor I (component B)
    CyQUANT™ Direct Cell Proliferation Assay Kit
    508nm
    Videos
    Documentos

    For Research Use Only. Not for use in diagnostic procedures.