Invitrogen™ Thyroxine (T4) Competitive ELISA Kit

Descripción

The Thyroxine (T4) ELISA quantitates Thyroxine (T4) in serum, plasma, urine, dried fecal extracts, or cell culture medium. The assay will exclusively recognize both natural and recombinant Thyroxine (T4). Principle of the method The Thyroxine (T4) solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, binding to the target on a different epitope from the capture antibody. A conjugated enzyme has been incorporated into the assay. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is inversely proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

La tiroxina es la hormona principal producida por la glándula tiroides. Las hormonas tiroideas, triyodotironina (T3) y tiroxina (T4), son hormonas a base de tirosina producidas por la glándula tiroides que son las principales responsables de la regulación del metabolismo. El yodo es necesario para la producción de T3 y T4. La principal forma de hormona tiroidea en la sangre es la tiroxina, que tiene una vida media más larga que la T3. La T4 se convierte en la T3 activa dentro de las células por medio de las deiodinasas (5'-iodinasa). Estas se procesan nuevamente mediante descarboxilación y desodinación para producir yodotironamina y tironamina. La producción de la hormona tiroidea se regula mediante la modulación de la tirotropina pituitaria (TSH) de la secreción de prohormona tiroxina por medio de la glándula tiroides y la regulación de la producción activa de triyodotironina. La tiroxina es idéntica en todas las especies.