Invitrogen™ Thrombopoietin Human ELISA Kit

Descripción

The Human Thrombopoietin (TPO) ELISA quantitates Hu TPO in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu TPO. Principle of the method The Human TPO solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

La trombopoyetina (TPO, THPO, ligando MPL, factor estimulante de colonias de megacariocitos) es responsable de la generación de plaquetas a través de su acción en las células hematopoyéticas comprometidas con linaje de megacariocitos. La unión de la trombopoyetina a su receptor, MPL (CD110), inicia una cascada de señales a través de las vías de señalización JAK/STAT, MAPK y PI3K/AKT que conduce a la proliferación y diferenciación de los megacariocitos y, en última instancia, a la producción de plaquetas (trombopoyesis). La trombopoyetina es producida constitutivamente por el hígado y tiene un peso molecular previsto de 38 kDa.