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Thermo Scientific™ Reactivos para kit de minipreparación de plásmidos GeneJET™

Components of the GeneJET Plasmid Miniprep Kit (K0502/K0503) that may be purchased separately.

32.04€ - 51.00€

Especificaciones

Para utilizar con (aplicación) DNA extraction
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Productos 6
Código de producto Marca Descripción Cantidad. Precio Cantidad y disponibilidad  
Código de producto Marca Descripción Cantidad. Precio Cantidad y disponibilidad  
10397979 Ver documentos Thermo Scientific™
R1223
Lysis Solution 70mL 44.75€
70 ml Ver productos alternativos
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Stock EU 8
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10669340 Ver documentos Thermo Scientific
R1213
70 ml 44.99€
70 ml Ver productos alternativos
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Stock EU 6
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10202510 Ver documentos Thermo Scientific
R1253
0.7mL 32.04€
0.70 ml Ver productos alternativos
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Stock EU 14
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10307989 Ver documentos Thermo Scientific™
R1233
Neutralization Solution 100mL 44.75€
100 ml Ver productos alternativos
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Stock EU 5
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10881361 Ver documentos Thermo Scientific
R1243
100mL 51.00€
100 ml Ver productos alternativos
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Stock EU 3
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10796031 Ver documentos Thermo Scientific
R1331
150mL 50.50€
150 ml Ver productos alternativos
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Stock EU 20
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Descripción

Descripción

The kit utilizes an exclusive silica-based membrane technology in the form of a convenient spin column. The kit recovers up to 20μg of high copy plasmid DNA per isolation procedure.

The Thermo Scientific GeneJET Plasmid Miniprep Kit utilizes an exclusive silica-based membrane technology in the form of a convenient spin column. The kit recovers up to 20 μg of high copy plasmid DNA per isolation procedure.

Highlights
  • Efficient - high yields of up to 20 μg of high quality plasmid DNA
  • Fast - procedure takes less than 14 minutes
  • Convenient - no phenol/chloroform extraction or alcohol precipitation required
  • Pure - purified DNA is immediately ready to use
Applications
Fast isolation of high purity plasmid DNA suitable for all conventional molecular biology procedures, including:
  • FastDigest or conventional restriction digestion
  • Automated fluorescent and radioactive sequencing
  • PCR
  • In vitro transcription
  • Transformation
Principle
A bacterial culture is harvested and lysed. The lysate is then cleared by centrifugation and applied on the silica column to selectively bind DNA molecules at a high salt concentration. The adsorbed DNA is washed to remove contaminants, and the pure plasmid DNA is eluted in a small volume of elution buffer or water. The purified DNA is ready for immediate use in all molecular biology procedures, such as fast and conventional digestion with restriction enzymes, PCR, in vitro transcription, transformation, and automated sequencing.

Components
  • Resuspension Solution
  • Lysis Solution
  • Neutralization Solution
  • Wash Solution (concentrated)
  • RNase A Solution
  • Elution Buffer
  • Spin Columns
  • Collection Tubes
  • Detailed Protocol

Hazardous: No
Quality Control:

  • The kit is tested for the isolation of pUC19 DNA from E. coli according to the protocol.
  • The quality of the isolated DNA is evaluated by spectrophotometric assay, agarose gel electrophoresis, digestion with FastDigest restriction enzymes, and automated sequencing.
Storage Condition: Ambient,-20°C

Fast isolation of high purity plasmid DNA suitable for all conventional molecular biology procedures, including:—FastDigest or conventional restriction digestion,—automated fluorescent and radioactive sequencing

Order Info

Orders placed Monday through Wednesday ship next day; Thursday and Friday orders ship the following Monday.

Especificaciones

Especificaciones

DNA extraction