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TFPI Recombinant Human Protein, His Tag, Invitrogen™ Sino Biological™
This recombinant protein was expressed from a DNA sequence encoding the human TFPI (NP_006278.1) (Met 1-Lys 282) fused to a C-terminal polyhistidine tag
169.00€ - 914.00€
Especificaciones
| Para utilizar con (aplicación) | Protein Expression and Isolation, Enzyme and Protein Activity Assay, Protein Assay Control, Reference Standards and Accessories |
|---|---|
| Peso molecular | 30.6kDa |
| Requisitos de almacenamiento | Store at -20°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed. |
| Concentración de endotoxinas | <1 EU/μg |
| Alias de gen | EPI, LACI, TFI, TFPI1 |
Descripción
Tissue Factor Pathway Inhibitor (Liporeotein-Associated Coagulation Inhibitor) (TFPI), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in protein studies such as enzymatic analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.- Activity: Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Anaspec Cat. No.27114), the IC50 value is <0.4nM, as measured in 100μL reaction mixture containing 1.25ng trypsin (Sigma Cat. No.T4799), 10μM substrate, 50mM Tris, 10mM CaCl2, 0.15M NaCl, 0.05% Brij-35, pH 7.5
- Formulation: Lyophilized in 140mM NaCl, 2.7mM KCl, 10mM Na2HPO4, 1.8mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80
- Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible
Especificaciones
| Protein Expression and Isolation, Enzyme and Protein Activity Assay, Protein Assay Control, Reference Standards and Accessories | |
| Store at -20°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed. | |
| EPI, LACI, TFI, TFPI1 | |
| TFPI | |
| Mammalian | |
| Lyophilized | |
| >85% by SDS-PAGE |
| 30.6kDa | |
| <1 EU/μg | |
| Tissue factor pathway inhibitor (liporeotein-associated coagulation inhibitor) | |
| His Tag | |
| Protease Inhibitors | |
| Proteinase Inhibitor |
For Research Use Only. Not for use in diagnostic procedures.