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Novus Biologicals™ Recombinant PreScission Protease Protein
Tienda Bio Techne Productos
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Cantidad:
100 UI
250 UI
Tamaño de la unidad:
cada uno
Descripción
The protease specifically recognizes a subset of sequences which include the core amino acid sequence Leu-Phe-Gln/Gly-Pro cleaving between the Gln and Gly residues. Substrate recognition and cleavage are likely to be dependent not only upon primary structural signals, but also upon the super structures of the fusion protein.
Especificaciones
Especificaciones
| Para utilizar con (aplicación) | SDS-PAGE, Enzyme Activity |
| Formulación | 50 mM Tris-HCl, pH 7.0, 150 mM NaCl, 1 mM EDTA, and 1 mM DTT. |
| Peso molecular | MolecularWeight-theroretical: 43 kDa |
| Cantidad | 100 UI |
| Requisitos de almacenamiento | Store at -20°C. Avoid freeze/thaw cycles. |
| Alias de gen | 3 C protease, Picornain 3 C, PSP |
| Propiedades del inhibidor | During cleavage reactions, it is recommended that samples be removed at various time points and analyzed by SDS-PAGE to estimate the yield, purity, and extent of digestion. The amount of PreScission Protease, temperature and length of incubation required for complete digestion of a given GST fusion partner may vary depending on the fusion partner. It works most effective at 4 C but can digest substrates (at room temperature. Optimal conditions for each fusion should be determined in pilot experiments. Digestion may be improved by adding TritonTM X-100, TweenTM 20 or NonidetTM P40 to a concentration of 0.01 %. Concentrations of these detergents up to 1 % do not inhibit PreScission Protease.) |
| Grado de pureza o calidad | Peptide affinity purified |
| Expresión | PreScission Protease |
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