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Descripción
Assay 1: Fully biologically active when compared to standard. Measured in a serum-free cell proliferation assay using human MCF-7 cells. The ED50 for this effect is 0.3-1.5 ng/mL, corresponding to a specific activity of > 6.7 x 10^5 U/mg. Assay 2: Fully biologically active when compared to standard. The ED50 as determined by the stimulation of protein synthesis using rat L6 myoblasts is less than 10 ng/mL, corresponding to a specific activity of > 1.0 x 10^5 U/mg.
Especificaciones
Especificaciones
| Para utilizar con (aplicación) | SDS-PAGE, Bioactivity |
| Formulación | Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.2. |
| ID de gen (Entrez) | 3479 |
| Peso molecular | MolecularWeight-theroretical: 9.1 kDa |
| Cantidad | 20 mg |
| Requisitos de almacenamiento | Store at -20°C to -70°C as supplied. After reconstitution, store at 2 to 8 C for 1 month and (at -20°C to -70°C for long term storage. Avoid repeated freeze/thaw cycles.) |
| Concentración de endotoxinas | Less than 0.01 EU/μg of IGF-I/IGF-1 AS determined by LAL method. |
| Alias de gen | IBP1, IGF1A, IGFI, IGF-I, IGF-IA, IGF-IB, insulin-like growth factor 1, insulin-like growth factor 1 (somatomedin C), insulin-like growth factor I, insulin-like growth factor IA, insulin-like growth factor IB, Mechano growth factor, MGF, somatomedin-C |
| Símbolo de gen | IGF1 |
| Reconstitución | Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL Apportion stock solutions into working aliquots and store (at <-20°C.) |
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