Thermo Scientific™ Pierce™ AKT In-Cell ELISA Kit
Measure relative total and phosphorylated Akt protein kinase levels using Akt antibodies detected by fluorescence or TMB substrate in these whole-cell microplate assays.
Marca: Thermo Scientific™ 62215
Detalles adicionales : CAS : 8-34-4 Peso : 1.81440kg
DescripciónAKT (protein kinase B, PKB, RAS-alpha) is a 57kDa serine/threonine kinase that plays an important role in diverse biological responses such as regulation of metabolism, cell survival and growth by phosphorylating multiple proteins. AKT is activated by insulin, PI3K, IGF1 and various other growth and survival factors. AKT promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including forkhead transcription factors, and caspase-9. There are three mammalian isoforms of Akt: AKT1 (PKB alpha), AKT2 (PKB beta) and AKT3 (PKB gamma) with AKT2 and AKT3 being approximately 82% identical with the AKT1 isoform. Each isoform has a pleckstrin homology (PH) domain, a kinase domain and a carboxy terminal regulatory domain. AKT was originally cloned from the retrovirus AKT8, and is a key regulator of many signal transduction pathways. The AKT pathway is a major target for cancer therpeutics where AKT signaling dysfunction has implicated in many types of cancer, including cancer syndromes known as phakomatoses.
|P31749, P31751, Q9Y243|
|Pierce AKT Colorimetric In-Cell ELISA Kit|
|10000, 207, 208|
|Blocking Buffer, Components included only in the target-specific kits:Antibody #1 and Antibody #2, Surfact-Amps X-100 (10% Detergent), 10mL, 20X Tris Buffered Saline, Surfact-Amps 20, Surfact-Amps X-100, HRP Conjugate, TMB Substrate, TMB Stop Solution, Janus Green Whole-Cell Stain, Elution Buffer, Thin Plate Seal Assembly|
|96 test kit|
|1 hr 20 min|
|Multi-component kit with HRP-conjugate|
|Colorimetric Microplate Reader|
|Supernatants (10,000 cells)|
|96 microplate wells of overnight-cultured cells|
|AKT1, AKT2, AKT3|