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Invitrogen™ Novex™ EGF Human ELISA Kit

Quantifies natural or recombinant human Epidermal Growth Factor (EGF) in serum, plasma, buffered solution, cell culture medium or urine

Marca:  Invitrogen™ KHG0061

Detalles adicionales : Peso : 0.14000kg

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Código de producto. 10405143

  • 532.00€ / 96 tests


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Descripción

Descripción

EGF belongs to a family of growth factors that bind to the 170 kDa EGF receptor. The EGF receptor ligands are synthesized as type I transmembrane proteins of 130 kDa with an N-terminal extension called the EGF module and a C-terminal fragment, also known as the cytoplasmic tail. EGF comprises nine EGF motives; only the one adjacent to the cell membrane is functional as an EGF receptor-binding domain. Due to proteolytic cleavage a soluble growth factor of 6 kDa containing the EGF module is released into the extracellular space (′ectodomain shedding′). EGF can be detected in most body fluids; the concentration is especially high in plasma, urine, saliva, and milk. Platelets as well as cells in various organs such as in brain, kidney, salivary gland, and stomach release EGF. As a mitogen for endothelial cells EGF also affects angiogenesis and plays a role in wound healing processes. Blocking the release of EGF receptor ligands inhibits growth and migration in several EGF receptor-dependent cell lines and greatly retards wound re-epithelialization due to impaired keratinocyte migration. Overexpression of one or more receptors and/or ligands is a feature of the majority of human carcinomas and epithelial cancers.
Especificaciones

Especificaciones

P01133
Biotin
1950
4 hr.
Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Biotinylated Detection Antibody, Streptavidin-HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
ELISA Kit
RUO
Plasma,Serum,Supernatant,Urine
Plasma,100 μL|Serum,100 μL|Supernatant,100 μL|Urine,10 μL
1 hr 20 min
Human
5.3%
HRP
3.9-250 pg/mL
Colorimetric Detection
Pre-coated plate, standard, detector antibody, Streptavidin-HRP, diluents, wash buffer, chromogen, stop solution, and plate covers.
Colorimetric Microplate Reader
EGF
96 Tests
<1 pg/mL
100μL
2-8°C
4 hr
EGF
4.4%
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For Research Use Only. Not for use in diagnostic procedures.