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Invitrogen™ Mouse IL-5 ELISA Kits
Measure levels of mouse interleukin-5 in mouse serum and culture supernatant samples using 96-well plates.
Marca: Invitrogen™ EMIL5
Detalles adicionales : Peso : 0.45000kg Transport : UN number : 2811 Chem class : 6.1 Pack group : III
Descripción
Assay IL-5 (mouse interleukin-5) levels in mouse serum and culture supernatant samples with our Thermo Scientific™ Mouse IL-5 ELISA Kits.
These mouse IL-5 ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interleukin-5 (IL-5) in samples at 50 to 100μL per well. Also included are calibrated recombinant protein standard, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. The antibody-pair kit consists of the matched IL-5 antibody pairs and IL-5 standard only. All components undergo quality control testing to minimize lot-to-lot variability.Target: Natural and recombinant mouse interleukin-5 (IL-5)
Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Measurement: Absorbance at 450nm minus absorbance at 550nm
Assay Range: 20 to 320pg/mL
Sensitivity: < 5pg/mL
Precision: Intra-assay CV < 10%; inter-assay CV < 10%
Sample Types: Cell culture supernatant, serum
Total Assay Time: 3.5 hours
Sample Size: 50μL
Specificity: No significant cross-reaction with with mouse IL-2, IL-3, IL-4, IL-6, IFNγ, GM-CSF, TNFα, rat IL-5, rabbit IL-5 or human IL-5
Includes:Complete Kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates. The Antibody Pair Kit (previously called a “MiniKit”) contains only matched antibodies and standard (no plates or detection reagents).
Especificaciones
| 20 to 320pg/mL | |
| Mouse IL-5 ELISA Kit | |
| Cell Culture Supernatant, Serum | |
| 96 microplate wells | |
| Absorbance at 450nm minus absorbance at 550nm | |
| 1 Plate Kit | |
| 3.5 hr. |
| <5pg/mL | |
| Colorimetric ELISA (TMB substrate) with Plates | |
| No significant cross-reaction with with mouse IL-2, IL-3, IL-4, IL-6, IFNg, GM-CSF, TNFa, rat IL-5, rabbit IL-5 or human IL-5 | |
| TMB Substrate Solution, 13mL, Adhesive Plate Covers, 6 ea, Stop Solution, 13mL, Anti-Mouse IL-5 Precoated 96-well Strip Plate, 1 ea, 30X Wash Buffer, 50mL, Anti-Mouse IL-5 HRP Conjugate Reagent, 12mL, Lyophilized Recombinant Mouse IL-5 Standard, 2 vials, Plate Reagent, 8mL, Standard Diluent, 12mL | |
| Intra-assay CV <10%; inter-assay CV <10% | |
| Natural and recombinant mouse interleukin-5 (IL-5) |