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KDEL Polyclonal Antibody, Invitrogen™
Rabbit Polyclonal Antibody
Marca: Invitrogen PA1013
Detalles adicionales : Peso : 0.01000kg
Descripción
PA1-013 detects KDEL from human, rat, mouse and hamster. PA1-013 has been successfully used in Western blot, immunofluorescence and immunoprecipitation procedures. By Western blot, this antibody detects a 57 kDa protein representing protein disulfide isomerase (PDI), a 78 kDa protein representing glucose regulated protein 70 kDa (GRP78) and an ∽94 kDa protein representing GRP94 in mouse myeloma cells. Immunofluorescence staining of GRP78 in mouse myeloma cells with PA1-013 results in diffuse cytoplasmic staining consistent with specific ER localization. PA1-013 immunoprecipitates PDI, GRP78, an ∽72 kDa protein which may be endoplasmic reticulum protein 72 kDa (ERp72), and an ∽94 kDa protein representing GRP94 from numerous rodent tissues. The PA1-013 immunogen is a synthetic peptide corresponding to residues C T(643) G E E D T S E K D E L(654) of rat GRP94. Reconstitute in 100 μL PBS to create a stock of 1 mg/mL.
The sequence Lys-Asp-Glu-Leu (KDEL) or a closely related sequence, is present at the carboxy-terminus of soluble endoplasmic reticulum (ER) resident proteins and some membrane proteins. 78 and 94 kDa glucose regulated proteins, GRP78 and GRP94 respectively, and protein disulfide isomerase (PDI) all share the C-terminal KDEL sequence. The presence of carboxy-terminal KDEL appears to be necessary for ER retention and appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor.Abbreviations: AP=Affinity Purification, ChIP=Chromatin IP, CM=Confocal Microscopy, DB=Dot Blot, EIA=ELISA, EM=Electron Microscopy, FC=Flow Cytometry, GS=Gel Supershift, IC=Immunocytochemistry, IF=Immunofluorescence, IH=Immunohistology/Immunohistochemistry, IP=Immunoprecipitation, IPK=IP Kinase Assay, KA=Kinase Assay, NEUT=Neutralization, PIA=Peptide Inhibition Assay, RIA=Radioimmunossay, TCA=T Cell Activation, WB=Western Blotting, Species: Am=Amphibian, Av=Avian, B=Bovine, Ca=Cat ,Ck=Chicken, D=Dog, Dr=Deer, Ds=Drosophila, El=Elk, Fg=Frog, Fs=Fish, Ft=Ferret, G=Goat, Gp=Guinea Pig, H=Horse, Hm=Hamster, Hu=Human, K=Kangaroo, Ms=Mouse, Nhp=Non-Human Primate, O=Ovine, P=Porcine, Rb=Rabbit, Rp=Reptile, Rt=Rat, Ys=Yeast, Xn=Xenopus, (wk)=cross reacts weakly
Especificaciones
KDEL | |
Polyclonal | |
Unconjugated | |
Hsp90b1 | |
78 kDa glucose-regulated protein; 8030486F04Rik; 94 kDa glucose-regulated protein; 94 kDa glucose-regulated protein {ECO:0000250; AL022860; AU019543; AW215843; baffled; Binding-immunoglobulin protein; BiP; D2Wsu141e; D2Wsu17e; ECGP; Endoplasmic reticulum chaperone BiP; endoplasmic reticulum lumenal Ca(2+)-binding protein grp78; endoplasmic reticulum resident protein 58; Endoplasmic reticulum resident protein 99; Endoplasmin; endoplasmin {ECO:0000250; endothelial cell (HBMEC) glycoprotein; EP58; epididymis luminal protein 35; epididymis secretory sperm binding protein Li 125m; epididymis secretory sperm binding protein Li 89n; ER lumen protein retaining receptor 1; ER lumen protein-retaining receptor 1; ER protein 58; ERD2; Erd2.1; ERp58; ERp99; glucose regulated protein, 78 kDa; glucose-regulated protein; glucose-regulated protein, 78kDa; gp96; gp96 homolog; GRP 78; Grp78; GRP-78; GRP94; GRP-94; HDEL; heat shock 70 kDa protein 5; heat shock 70kD protein 5; heat shock 70kD protein 5 (gl | |
Rabbit | |
Antigen affinity chromatography | |
RUO | |
100689305, 100750912, 100773331, 10945, 14828, 22027, 25617, 3309, 361577, 362862, 68137, 7184 | |
-20° C, Avoid Freeze/Thaw Cycles | |
Lyophilized |
Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot | |
1 mg/mL | |
antibody with no preservative | |
P06761, P08113, P11021, P14625, P20029, P24390, Q569A6, Q66HD0, Q99JH8 | |
Hsp90b1, HSPA5, Kdelr1 | |
Synthetic peptide corresponding to residues C T(643) G E E D T S E K D E L(654) of rat GRP94. | |
100 μg | |
Primary | |
Hamster, Human, Mouse, Rat | |
Antibody | |
IgG |