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Invitrogen™ pcDNA™3.1 Directional TOPO™ Expression Kit

Linearized, topoisomerase I-activated pcDNA3.1D/V5-His-TOPO vector for 5-minute directional cloning and subsequent high-level expression

Marca:  Invitrogen™ K490001

Detalles adicionales : Peso : 0.00500kg

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Código de producto. 10605583

  • 834.00€ / cada uno


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Descripción

Descripción

TOPO TA Cloning kits
• Alta eficiencia
• 'One-step'
Especificaciones

Especificaciones

Directional TOPO®
Constitutive Expression
Constitutive
CMV
20 reactions
His Tag (6x), V5 Epitope Tag
Box 1 (Store at -20°C):
• 20 µl pcDNA™3.1D/V5-His-TOPO® (15–20 ng/µl)
• 10 µl dNTP Mix
• 50 µl Salt Solution
• 1 ml Sterile Water
• 20 µl T7 Sequencing Primer (0.1 µg/µl)
• 20 µl BGH Reverse Sequencing Primer (0.1 µg/µl)
• 10 µl Control PCR Template (0.1 µg/µl)
• 10 µl Control PCR Primers (0.1 µg/µl each)
• 10 µl Expression Plasmid (0.5 µg/µl)

Box 2 (Store at -80°C):
• 6 ml S.O.C. Medium (may be stored at 4°C or room temperature)
• 21 × 50 µl TOP10 Cells
• 50 µl pUC19 Control DNA
TOPO®, pcDNA™
Transfection
Geneticin® (G-418)
Directional TOPO Vectors, pcDNA
Videos
Documentos

For Research Use Only. Not for use in diagnostic procedures.