Invitrogen™ IL-2 Bovine ELISA Kit

Descripción

The Bovine Interleukin 2 (Bov IL-2) ELISA quantitates Bov IL-2 in bovine serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Bov IL-2. Principle of the method The Bovine IL-2 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

La interleucina 2 (IL-2) es una citoquina inmunomoduladora importante para la proliferación de células T activadas, la diferenciación de células B, células asesinas naturales, monocitos y macrófagos. La IL-2 envía señales a través del receptor de IL-2 (IL-2R), un complejo de proteínas heterotriméricas cuya cadena gamma también es compartida por la interleucina 4 (IL-4) y la interleucina 7 (IL-7). La expresión del gen IL-2 en los timocitos maduros es monoalélica, lo que representa un modo de regulación inusual para controlar la expresión precisa de un solo gen. La alteración selectiva de un gen similar al IL-2 en ratones conduce a una enfermedad ulcerosa similar a la colitis que sugiere un papel esencial de este gen en la respuesta inmunológica a los estímulos antigénicos.