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Goat anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 647, Invitrogen™
Goat Polyclonal Secondary Antibody
171.00€ - 373.00€
Especificaciones
Antígeno | Human IgG (H+L) Cross-Adsorbed |
---|---|
Concentración | 2 mg/mL |
Aplicaciones | Immunocytochemistry, Western Blot |
Clasificación | Polyclonal |
Conjugado | Alexa Fluor Plus 647 |
Código de producto | Marca | Cantidad | Precio | Cantidad y disponibilidad | |||||
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Código de producto | Marca | Cantidad | Precio | Cantidad y disponibilidad | |||||
17206553
|
Invitrogen
A48279TR |
100 μg |
171.00€
100 microgramos |
Fecha estimada de envío: 24-05-2024 Inicie sesión para ver el stock |
Por favor, inicie sesión para comprar este producto. ¿No tiene usuario web? Regístrese hoy mismo. | ||||
17296543
|
Invitrogen
A48279 |
1 mg |
373.00€
1 miligramo |
En stock Inicie sesión para ver el stock |
Por favor, inicie sesión para comprar este producto. ¿No tiene usuario web? Regístrese hoy mismo. | ||||
Descripción
To minimize cross-reactivity, the goat anti-human IgG whole antibodies have been cross-adsorbed against serum proteins from bovine, mouse, and rabbit to increase specificity of the antibody resulting in less background staining and cross-reactivity. The secondary antibody solution is passed through a column matrix containing immobilized serum proteins from potentially cross-reactive species. Only nonspecific-binding secondary antibodies are captured in the column, and the highly specific secondaries flow through. Further passages through additional columns result in highly cross-adsorbed preparations of secondary antibody. The benefits of these extra steps are apparent in multiplexing/multicolor-staining experiments where there is potential cross-reactivity with other primary antibodies or in tissue/cell fluorescent staining experiments where there may be the presence of endogenous immunoglobulins. Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment to eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining. Because staining protocols vary with application, the appropriate dilution of antibody should be determined empirically. This antibody binds to heavy chains on human IgG and light chains on all human immunoglobulins. This antibody does not bind non-immunoglobulin human serum proteins or serum proteins/IgG from bovine, mouse, and rabbit.
Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.Especificaciones
Human IgG (H+L) Cross-Adsorbed | |
Immunocytochemistry, Western Blot | |
Alexa Fluor Plus 647 | |
Goat | |
Human | |
Gamma Immunoglobins Heavy and Light chains | |
Secondary | |
4° C, store in dark |
2 mg/mL | |
Polyclonal | |
Liquid | |
RUO | |
proprietary buffer with 0.016% Bromonitrodioxane, 0.016% Methylisothiazolone; pH 6.5 | |
IgG | |
Affinity chromatography |