Invitrogen™ Human Cyclophilin ELISA Kit
Human Cyclophilin quantitates human Cyclophilin in serum, plasma, supernatant.
Marca: Invitrogen™ EH138RBX10
Detalles adicionales : Peso : 2.50000kg
Human Cyclophilin quantitates human Cyclophilin in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human Cyclophilin.Immunophilins are a family of soluble cytosolic receptors capable of binding to one of two major immunosuppressant agents - cyclosporin A (CsA) or FK506. Proteins that bind FK506 are termed FK506 Binding Proteins (FKBPs) and those that bind cyclosporin A are called cyclophilins (CyP). Both CyP:CsA and FKBP:FK506 complexes have been shown to inhibit calcineurin, a calcium and calmodulin dependent protein phosphatase which has been implicated as an important signaling enzyme in T-cell activation, providing a possible mechanism of immunosuppression by CsA and FK506. Immunophilins function as peptidyl prolyl cis-trans-isomerases (PPIase) whose activity is inhibited by their respective immunosuppressant compounds. As PPIase's, immunophilins accelerate folding of some proteins both in vivo and in vitro by catalyzing slow steps in the initial folding and rearrangement of proline containing proteins. Within the cyclophilin family, there are several different proteins which show a high degree of homology including CyPA, CyPB and CyPC. CyPA, also termed CyP-18, is the most abundant and ubiquitous cyclophilin found in all vertebrate tissues and is present in T-cells.
|2700098C05; Cphn; CYCA; cyclophilin; Cyclophilin A; Cyclosporin A-binding protein; CyP A; CyP-18; CypA; CyP-A; CYPH; EC 22.214.171.124; epididymis secretory sperm binding protein Li 69 p; HEL-S-69 p; HGNC:9253; I79_011869; MGC117158; MGC12404; MGC23397; p1B15; p31; Peptidyl-prolyl cis-trans isomerase A; Peptidyl-prolyl cis-trans isomerase A, N-terminally processed; peptidylprolyl isomerase A; peptidylprolyl isomerase A (cyclophilin A); PPIA; PPIase; PPIase A; rotamase; Rotamase A; SP18; T cell cyclophilin|
|Colorimetric Microplate Reader|
|Research Use Only|
|2°C to 8°C|
|1 hr 20 min|
|2.05 to 500 ng/mL|
|Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers|
|10 x 96 Tests|
|Plasma, 50 μL; Serum, 50 μL; Supernatant, 50 μL|
|4 hr 45 min|