GAD67 Polyclonal Antibody, Invitrogen™

Descripción

Recommended positive controls: NT2D1, IMR32, Jurkat, HeLa. Predicted reactivity: Mouse (97%), Rat (97%), Dog (97%), Cat (97%), Pig (97%), Chicken (94%), Chimpanzee (99%), Bovine (97%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

GAD-65 and GAD-67 are members of the group II decarboxylase family of proteins and are responsible for catalyzing the rate limiting step in the production of GABA (gamma-aminobutyric acid) from L-glutamic acid. Although both GADs are found in the brain, GAD-65 localizes to synaptic vesicle membranes in nerve terminals, while GAD-67 is distributed throughout the cell. GAD-67 is responsible for the basal levels of GABA synthesis. In the case of a heightened demand for GABA in neurotransmission, GAD-65 will transiently activate to assist in GABA production. The loss of GAD-65 is detrimental and can impair GABA neurotransmission, however the loss of GAD-67 is lethal. Due to alternative splicing, two isoforms exist for GAD-67: the predominant GAD-67 form and the minor GAD-25 form.