Thermo Scientific™ Kit de ligadura rápida de ADN
Enable fast sticky-end or blunt-end DNA ligation in only 5 minutes at room temperature.
Marca: Thermo Scientific™ K1423
Detalles adicionales : Peso : 1.58760kg
DescripciónThe kit contains T4 DNA ligase and a specially formulated 5X rapid ligation buffer optimized for fast and efficient DNA ligation. Fast ligation efficiency is equal to that obtained with T4 DNA ligase in a standard 1 hour ligation. The ligation reaction mixture can be used directly for bacterial transformation with the TransformAid Bacterial Transformation Kit or with other conventional transformation procedures.
- Fast—ligation of either sticky- or blunt-end DNA in only 5 minutes
- Convenient—reaction mixture can be used directly for bacterial transformation
Routine cloning experiments; Blunt-end cloning; Library construction; TA cloning.
Lyophilized bacterial strains GM2163 (†M0099) and JM107 (†M0109) can be provided free of charge upon request, with any purchase. Prior to electroporation it is necessary to inactivate T4 DNA ligase (1) by chloroform extraction.