Invitrogen™ DAN (PARN) Mouse ELISA Kit

Descripción

The Mouse DAN (PARN) ELISA quantitates Ms DAN in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms DAN. Principle of the method The Mouse DAN solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

La proteína codificada por este gen es una 3′-exorribonucleasa, similar a la familia RNasa D de 3′-exonucleasas. Prefiere poli(A) como sustrato, por lo tanto, degrada eficientemente las colas poli(A) de los ARNm. La degradación exonucleolítica de la cola poli(A) es a menudo el primer paso en la descomposición de los ARNm eucarióticos. Esta proteína también está implicada en el silenciamiento de ciertos ARNm maternos durante la maduración de los ovocitos y el desarrollo embrionario temprano, así como en la degradación mediada por mutaciones terminadoras (NMD) de los ARNm que contienen codones de parada prematuros. Alternativamente, se han encontrado variantes de transcripción empalmadas que codifican diferentes isoformas para este gen.