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CD24 Monoclonal Antibody (M1/69), Super Bright™ 645, eBioscience™, Invitrogen™
Rat Monoclonal Antibody
en la oferta
Especificaciones
Antígeno | CD24 |
---|---|
Clon | M1/69 |
Concentración | 0.2 mg/mL |
Aplicaciones | Flow Cytometry |
Clasificación | Monoclonal |
Descripción
Description: The SN3 monoclonal antibody reacts with the human CD24 molecule, also known as Heat Stable Antigen (HSA). This 35-50 kDa molecule is anchored in the plasma membrane via phosphatidylinositol and is expressed by erythrocytes, thymocytes, peripheral lymphocytes and myeloid lineage. It has been reported that P-selectin (CD62P) binds to CD24. Applications Reported: This eBioSN3 (SN3 A5-2H10) antibody has been reported for use in flow cytometric analysis. Applications Tested: This eBioSN3 (SN3 A5-2H10) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.5 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Super Bright 645 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 645 nm. We recommend using a 660/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome. When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Staining Buffer (Product No. SB-4400) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation.
CD24 is a two-chain glycosylphosphatidylinositol (GPI)-anchored glycoprotein expressed at multiple stages of B-cell development, beginning with the bone marrow pro-B-cell compartment and continuing through mature, surface Ig positive B-cells. Plasma cell expression is very low or negative. CD24 is also expressed on the majority of B-lineage acute lymphoblastic leukemias, B-cell CCLs and B-cell non-Hodgkin's lymphomas. CD24 may play a role in regulation of B-cell proliferation and maturation, and control of autoimmunity.Especificaciones
CD24 | |
0.2 mg/mL | |
Monoclonal | |
Liquid | |
RUO | |
P24807 | |
Cd24a | |
Primary | |
4° C, store in dark, DO NOT FREEZE! | |
Cd24a |
M1/69 | |
Flow Cytometry | |
Super Bright 645 | |
Rat | |
Mouse | |
12484 | |
IgG2b κ | |
Affinity chromatography | |
Antibody |