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Goat anti-Mouse IgG (H+L) Cross-Adsorbed, Alexa Fluor 488, Polyclonal, Secondary Antibody, Invitrogen™

Goat Polyclonal Secondary Antibody

Marca:  Invitrogen A11001

Detalles adicionales : Peso : 0.00454kg

Código de producto. 10256302

  • 235.00€ / 500 microlitros


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Descripción

Descripción

To minimize cross-reactivity, these goat anti-mouse IgG whole antibodies have been cross-adsorbed against human IgG and human serum. Cross-adsorption or pre-adsorption is a purification step to increase specificity of the antibody resulting in higher sensitivity and less background staining. The secondary antibody solution is passed through a column matrix containing immobilized serum proteins from potentially cross-reactive species. Only the nonspecific-binding secondary antibodies are captured in the column, and the highly specific secondaries flow through. The benefits of this extra step are apparent in multiplexing/multicolor-staining experiments (e.g., flow cytometry) where there is potential cross-reactivity with other primary antibodies or in tissue/cell fluorescent staining experiments where there are may be the presence of endogenous immunoglobulins. For a highly cross-adsorbed secondary antibody equivalent, please see product Cat. No. A11029.Alexa Fluor dyes are among the most trusted fluorescent dyes available today. Invitrogen™ Alexa Fluor 488 dye is a bright, green-fluorescent dye with excitation ideally suited to the 488 nm laser line. For stable signal generation in imaging and flow cytometry, Alexa Fluor 488 dye is pH-insensitive over a wide molar range. Probes with high fluorescence quantum yield and high photostability allow detection of low-abundance biological structures with great sensitivity. Alexa Fluor 488 dye molecules can be attached to proteins at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection. The degree of labeling for each conjugate is typically 2-8 fluorophore molecules per IgG molecule; the exact degree of labeling is indicated on the certificate of analysis for each product lot.Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining. Because staining protocols vary with application, the appropriate dilution of antibody should be determined empirically. For the fluorophore-labeled antibodies a final concentration of 1-10μg/mL should be satisfactory for most immunohistochemistry and flow cytometry applications.
Especificación

Especificación

Mouse IgG (H+L) Cross Adsorbed
Polyclonal
Alexa Fluor 488
Liquid
Gamma Immunoglobins Heavy and Light chains
Purified
RUO
Secondary Antibody
Polyclonal
Flow Cytometry, Immunocytochemistry, Immunofluorescence
2mg/mL
PBS with 5mM sodium azide; pH 7.5
Goat
IgG
500μL
4° C, store in dark
Secondary
Murine
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