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Bub3 Mouse, Unlabeled, Clone: 31, BD
Mouse Monoclonal Antibody
Marca: BD Biosciences 611731
Detalles adicionales : Peso : 0.01000kg
Descripción
Accurate chromosome segregation requires that all pairs of sister chromatids become appropriately attached to mitotic spindles before the onset of anaphase. Cell cycle checkpoints monitor kinetochore-microtubule interactions, so that cell cycle progression can be delayed until proper chromosome attachments are formed. In yeast, Bub1-3 genes are required for proper mitotic delay in response to unattached kinetochores. In mammals, the homologues to yeast Bub1 and Bub3 form a complex that binds kinetochores and has protein kinase activity. Bub3 contains four WD repeats, three in the N-terminus and one in the C-terminus, and a central Bub1-binding domain. During prophase and prometaphase, Bub3 localizes to the kinetochore before attachment to microtubules. In addition, taxol-induced formation of lagging chromosomes due to a delay of cell cycle progression increases the level of Bub3 co-localized with kinetochores, while correctly aligned chromosomes found in metaphase do not exhibit this co-localization. Thus, Bub3, in association with Bub1, may be important for sensing kinetochore attachment to microtubules during the prometaphase to metaphase transition.
Host Species: Mouse
Clone: 31
Isotype: IgG1
Species Reactivity [for Features Main]: Human
Immunogen: Human Bub3 aa. 4-16
Bioimaging, Immunofluorescence, Western Blotting
Especificaciones
| Bub3 | |
| Monoclonal | |
| 250μg/mL | |
| Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide. | |
| Human Bub3 aa. 4-16 | |
| 150 μg | |
| Cell Biology | |
| Human, Mouse, Rat | |
| IgG1 |
| Western Blot | |
| 31 | |
| Unconjugated | |
| Mouse | |
| Affinity Purified | |
| RUO | |
| Primary | |
| Store undiluted at -20°C. |
For Research Use Only.