pH variation with Temperature

   Temperature °C   pH      Temperature °C   pH  
 pH 2 Buffer (glycine)  202,000  pH 6 Buffer (phosphate)  156,022
 (J/2800)  252,101  (J/2840)  206,000
         255,976
         305,957
 pH 3 Buffer (phthalate)  02,910   355,937
 (J/2810)  52,912       
  102,918       
  152,919  pH 7 Buffer (phosphate)  07,115
  202,924  (J/2850, J/2855 colour  57,083
  252,932  coded, B/4765 tablets)  107,056
  302,936   157,032
  352,944   207,012
  402,953   256,994
  452,962   306,983
  502,973   356,975
  552,984   406,968
  602,998   456,970
         506,970
         556,970
 pH 4 Buffer (phthalate)  04,007   606,974
 (J/2820, J/2825 colour  54,002       
 coded, B/4760 tablets)  103,999       
  154,000  pH 8 Buffer (borate)  158,017
  204,003  (J/2860, J/2860C)  208,000
  254,008   257,990
  304,016   307,975
  354,025   357,967
  404,036       
  454,050       
  504,068  pH 9,2 Buffer (borate)  09,431
  554,084  (J/2870, B/4770 tablets)  59,361
  604,101   109,302
         159,247
         209,200
 pH 5 Buffer (phthalate)  04,998   259,155
 (J/2830)  54,980   309,114
  104,969       
  154,958   359,079
  204,955   409,046
  254,955   459,017
  304,957   508,993
  354,963   558,968
  404,974   608,944
  454,989       
  505,006       
  555,028       
  605,052       
Temperature °CpH
pH 2 Buffer (glycine)
(J/2800)
20 2,000
25 2,101
pH 3 Buffer (phthalate)
(J/2810)
0 2,910
5 2,912
10 2,918
15 2,919
20 2,924
25 2,932
30 2,936
35 2,944
40 2,953
45 2,962
50 2,973
55 2,984
60 2,998
pH 4 Buffer (phthalate)
(J/2820, J/2825 colour coded, B/4760 tablets)
0 4,007
5 4,002
10 3,999
15 4,000
20 4,003
25 4,008
30 4,016
35 4,025
40 4,036
45 4,050
50 4,068
55 4,084
60 4,101
pH 5 Buffer (phthalate)
(J/2830)
0 4,998
5 4,980
10 4,969
15 4,958
20 4,955
25 4,955
30 4,957
35 4,963
40 4,974
45 4,989
50 5,006
55 5,028
60 5,052
Temperature °CpH
pH 6 Buffer (phosphate)
(J/2840)
15 6,022
20 6,000
25 5,976
30 5,957
35 5,937
pH 7 Buffer (phosphate)
(J/2850, J/2855 colour coded, B/4765 tablets)
0 7,115
5 7,083
10 7,056
15 7,032
20 7,012
25 6,994
30 6,983
35 6,975
40 6,968
45 6,970
50 6,970
55 6,970
60 6,974
pH 8 Buffer (borate)
(J/2860, J/2860C)
15 8,017
20 8,000
25 7,990
30 7,975
35 7,967
pH 9,2 Buffer (borate)
(J/2870, B/4770 tablets)
0 9,431
5 9,361
10 9,302
15 9,247
20 9,200
25 9,155
30 9,114
35 9,079
40 9,046
45 9,017
50 8,993
55 8,968
60 8,944

pH Ranges of Selected Biological Buffers (0.1M, 25°C)


pH Ranges of Selected Biological Buffers (0.1M, 25°C)