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Smad3, p Ser423, p Ser425 Antibody, Novus Biologicals™

Código de producto. 18791464 Tienda Bio Techne Productos
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Cantidad:
0.025 mg
Tamaño de la unidad:
25 microgramos
Código de producto. Cantidad unitSize
18791464 0.025 mg 25 microgramos
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Código de producto. 18791464

Marca: Novus Biologicals NBP177836SS

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Descripción

Rabbit Polyclonal Antibody

Smad3 (p Ser423, p Ser425) Polyclonal antibody specifically detects Smad3 (p Ser423, p Ser425) in Human, Mouse samples. It is validated for Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Single Cell Western.
TRUSTED_SUSTAINABILITY

Especificaciones

Antígeno Smad3 (p Ser423, p Ser425)
Aplicaciones Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Western Blot
Clasificación Polyclonal
Concentración 1.1 mg/ml
Conjugado Unconjugated
Dilución Western Blot 1:2000-1:20000, Flow Cytometry 1:10-1:1000, ELISA 1:15000-1:30000, Immunohistochemistry 1:500, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin 1:10-1:500, Single Cell Western 100 ug/ml
Formulación 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% Sodium Azide
N.º de referencia del gen NC_000015.9
Alias de gen DKFZp586N0721, DKFZp686J10186, hMAD-3, hSMAD3, HsT17436, JV15-2MGC60396, MAD homolog 3, mad protein homolog, MAD, mothers against decapentaplegic homolog 3, MAD, mothers against decapentaplegic homolog 3 (Drosophila), Mad3, MADH3mad homolog JV15-2, mothers against decapentaplegic homolog 3, Mothers against DPP homolog 3, pSMAD, SMA- and MAD-related protein 3, SMAD 3, SMAD family member 3HSPC193, SMAD, mothers against DPP homolog 3, SMAD, mothers against DPP homolog 3 (Drosophila), Smad3
Símbolos de los genes SMAD3
Especie del huésped Rabbit
Inmunógeno Smad3 [p Ser423, p Ser425] Antibody was prepared from whole rabbit serum produced by repeated immunizations with a dual phosphorylated synthetic peptide corresponding to a c-terminal region with Serine 423 and Serine 425 of human Smad3 protein. (Uniprot: P84022)
Peso molecular del antígeno 48.08 kDa
Método de purificación Affinity Purified
Cantidad 0.025 mg
Estado normativo RUO
Primario o secundario Primary
ID de gen (Entrez) 4088
Especificidad de la prueba This affinity-purified antibody is directed against the phosphorylated form of human Smad3 protein at the pS423 and pS425 residues. The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide. Reactivity occurs against human Smad3 pS423 and pS425 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human Smad3 is minimal by ELISA and western blot. Expect reactivity against phosphorylated Smad1 and Smad5. Negligible reactivity is seen against other phosphorylated Smad family members. A BLAST analysis was used to suggest cross reactivity with Smad3 from human, Xenopus species laevis, Xenopus species tropicalis, zebrafish, rat, mouse, swine, bovine and chicken based on 100% sequence homology with the immunogen. Reactivity against homologues from other sources is not known.
Especies diana Human, Mouse, Rat, Pig, Bovine, Chicken, Xenopus species, Zebrafish
Contenido y almacenamiento Store at -20°C short term. Aliquot and store at -80°C long term. Avoid freeze-thaw cycles.
Isotype IgG
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