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Smad3, p Ser423, p Ser425 Antibody, Novus Biologicals™
Rabbit Polyclonal Antibody
Marca: Novus Biologicals NBP1-77836SS
Código de producto. 18791464
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262.00€
247.80€ / 25 microgramos
Ahorro 14.20€
5% Descuento
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Descripción
Smad3 (p Ser423, p Ser425) Polyclonal antibody specifically detects Smad3 (p Ser423, p Ser425) in Human, Mouse samples. It is validated for Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Single Cell Western.
Especificaciones
| Smad3 (p Ser423, p Ser425) | |
| Polyclonal | |
| Unconjugated | |
| 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% Sodium Azide | |
| DKFZp586N0721, DKFZp686J10186, hMAD-3, hSMAD3, HsT17436, JV15-2MGC60396, MAD homolog 3, mad protein homolog, MAD, mothers against decapentaplegic homolog 3, MAD, mothers against decapentaplegic homolog 3 (Drosophila), Mad3, MADH3mad homolog JV15-2, mothers against decapentaplegic homolog 3, Mothers against DPP homolog 3, pSMAD, SMA- and MAD-related protein 3, SMAD 3, SMAD family member 3HSPC193, SMAD, mothers against DPP homolog 3, SMAD, mothers against DPP homolog 3 (Drosophila), Smad3 | |
| Rabbit | |
| 48.08 kDa | |
| 0.025 mg | |
| Primary | |
| This affinity-purified antibody is directed against the phosphorylated form of human Smad3 protein at the pS423 and pS425 residues. The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide. Reactivity occurs against human Smad3 pS423 and pS425 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human Smad3 is minimal by ELISA and western blot. Expect reactivity against phosphorylated Smad1 and Smad5. Negligible reactivity is seen against other phosphorylated Smad family members. A BLAST analysis was used to suggest cross reactivity with Smad3 from human, Xenopus species laevis, Xenopus species tropicalis, zebrafish, rat, mouse, swine, bovine and chicken based on 100% sequence homology with the immunogen. Reactivity against homologues from other sources is not known. | |
| Store at -20°C short term. Aliquot and store at -80°C long term. Avoid freeze-thaw cycles. |
| Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Western Blot | |
| 1.1 mg/ml | |
| Western Blot 1:2000-1:20000, Flow Cytometry 1:10-1:1000, ELISA 1:15000-1:30000, Immunohistochemistry 1:500, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin 1:10-1:500, Single Cell Western 100 ug/ml | |
| NC_000015.9 | |
| SMAD3 | |
| Smad3 [p Ser423, p Ser425] Antibody was prepared from whole rabbit serum produced by repeated immunizations with a dual phosphorylated synthetic peptide corresponding to a c-terminal region with Serine 423 and Serine 425 of human Smad3 protein. (Uniprot: P84022) | |
| Affinity Purified | |
| RUO | |
| 4088 | |
| Human, Mouse, Rat, Pig, Bovine, Chicken, Xenopus species, Zebrafish | |
| IgG |
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