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R&D Systems™ Recombinant Human PDGF-AB (single chain) Protein, CF

Código de producto. 18744564
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Cantidad:
100 μg
10 μg
1 mg
250 μg
500 μg
50 μg
Tamaño de la unidad:
1 miligramo
10 microgramos
100 microgramos
250 microgramos
50 microgramos
500 microgramos
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Código de producto. 18744564

Marca: R&D Systems™ 11471AB500

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Recombinant Protein

Measured in a cell proliferation assay using NR6R-3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED50 for this effect is 2.00-20.0 ng/mL.
TRUSTED_SUSTAINABILITY

Specifications

Número de acceso NP_001382295.1 & P01127.1
Para utilizar con (aplicación) Bioactivity
Formulación Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with Trehalose.
ID de gen (Entrez) 5154
Peso molecular MolecularWeight-observed: 28-37 kDa, under reducing conditions., MolecularWeight-theroretical: 26 kDa
Cantidad 500 μg
Fuente Human embryonic kidney cell, HEK293-derived human PDGF-AB protein Human PDGFA (Ser87-Arg196) (Cys123Ala, Cys132Ala) (N-terminus) Accession # NP_001382295.1 linker Human PDGFB (Ser82-Thr190) (Cys123Ala, Cys132Ala) (N-terminus) Accession # P01127.1 (C-terminus)
Requisitos de almacenamiento Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month, 2 to 8°C under sterile conditions after reconstitution. 3 months, -20 to -70°C under sterile conditions after reconstitution.
Concentración de endotoxinas <0.10 EU per 1 μg of the protein by the LAL method.
Conjugado Unconjugated
Reconstitución Reconstitute 10 μg size at 100 μg/mL in 4 mM HCl. Reconstitute all the other sizes at 250 μg/mL in 4 mM HCl.
Grado de pureza o calidad >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
Expresión PDGF-AB
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