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R&D Systems™ Recombinant Human FGF basic/FGF2 Protein, Animal-Free
Intended for preclinical researchers who may transition to GMP FGF basic/FGF2 for their clinical work
Marca: R&D Systems™ BT-FGFB-AFL-01M
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Descripción
Measured in a cell proliferation assay using NR6R-3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED50 for this effect is 0.100-1.00 ng/mL.The specific activity of Recombinant Human FGF basic/FGF2 is >8.0 x 105 IU/mg, which is calibrated against the human FGF basic/FGF2 WHO International Standard (NIBSC code: 90/712).
Especificaciones
| NP_001997.5 | |
| Lyophilized from a 0.2 μm filtered solution in HEPES and Sodium Sulfate with Trehalose. | |
| M.W. (Observed): 18 kDa, under reducing conditions., M.W. (Theroretical): 16 kDa | |
| E. coli-derived human FGF basic/FGF2/bFGF protein Ala135-Ser288 Produced using non-animal reagents in an animal-free laboratory. | |
| <0.01 EU per 1 μg of the protein by the LAL method. | |
| Unconjugated | |
| >97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining. |
| Bioactivity | |
| 2247 | |
| 1 mg | |
| Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution. | |
| Basic fibroblast growth factor, basic fibroblast growth factor bFGF, bFGF, FGFBprostatropin, fibroblast growth factor 2 (basic), HBGF-2, heparin-binding growth factor 2 | |
| Reconstitute 25 μg size at 250 μg/mL and others at 500 μg/mL in sterile water. | |
| FGF basic/FGF2/bFGF |
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Título del producto
R&D Systems™ Recombinant Human FGF basic/FGF2 Protein, Animal-Free > 1 mg
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