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R&D Systems™ Recombinant Human FGF basic/FGF2 Protein, Animal-Free
Descripción
Measured in a cell proliferation assay using NR6R-3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED50 for this effect is 0.100-1.00 ng/mL.The specific activity of Recombinant Human FGF basic/FGF2 is >8.0 x 105 IU/mg, which is calibrated against the human FGF basic/FGF2 WHO International Standard (NIBSC code: 90/712).
Especificaciones
Especificaciones
| Número de acceso | NP_001997.5 |
| Para utilizar con (aplicación) | Bioactivity |
| Formulación | Lyophilized from a 0.2 μm filtered solution in HEPES and Sodium Sulfate with Trehalose. |
| ID de gen (Entrez) | 2247 |
| Peso molecular | M.W. (Observed): 18 kDa, under reducing conditions., M.W. (Theroretical): 16 kDa |
| Cantidad | 25 μg |
| Fuente | E. coli-derived human FGF basic/FGF2/bFGF protein Ala135-Ser288 Produced using non-animal reagents in an animal-free laboratory. |
| Requisitos de almacenamiento | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution. |
| Concentración de endotoxinas | <0.01 EU per 1 μg of the protein by the LAL method. |
| Alias de gen | Basic fibroblast growth factor, basic fibroblast growth factor bFGF, bFGF, FGFBprostatropin, fibroblast growth factor 2 (basic), HBGF-2, heparin-binding growth factor 2 |
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