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Eurofins DiscoverX PKCα Protein, Active
Descripción
Protein kinase Ca (PKCa) is one of the major protein kinase C isoforms of a family of serine/threonine protein kinases. Breakdown of phosphatidylinositol leads to formation of diacylglycerol and inositol triphophate, the former acting synergistically with Ca2+ to activate protein kinase C (PKC), resulting in translocation of the enzyme from cytosol to the membrane. Phosphorylation of proteins by PKC has been implicated as a pivotal regulatory element in signal transduction, cellular regulation, and with a high affinity to phorbol ester tumor promoters.
GenBank X52479
Especificaciones
Especificaciones
| Formulación | mg/mL of enzyme in 20 mM Tris/HCl pH 7.5, 5% glycerol, 10 mM benzamidine, 1 mM PMSF, 1 mM EGTA, 1 mM EDTA, 0.02% Triton X-100, 0.1% 2-mercaptoethanol. Frozen solution. |
| Peso molecular | 78 kDa |
| Cantidad | 250 μg |
| Especie | Human |
| Recombinante | Recombinant |
| Etiqueta de proteína | N-terminal 6His |
| Sistema de expresión | Recombinant enzyme expressed in Sf21 insect cells |
| Forma de proteína | Full-length |
| Grado de pureza o calidad | ≥78% |
| Expresión | PKC alpha, active |
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