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DescripciónEpitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Green Fluorescent Protein (GFP), from the jellyfish Aequorea victoria, has quickly become a versatile marker for monitoring physiological processes, visualizing protein localization, and detecting transgenic expression, and a powerful research tool for assessing gene expression and subcellular protein distribution in fixed or living cells. GFP is excited by and brightly fluoresces when exposed to UV or blue light. This feature makes it ideal as a marker for use in fluorescence microscopy, cytometry, tagging fusion proteins, and assaying transcriptional regulation from gene promoters in vivo. Numerous GFP variants with enhanced and shifted emission spectra (blue, green, and yellow) have been developed through amino acid substitutions at specific residues.
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|Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot|
|PBS with 5mM sodium azide; pH 7.2|
|GFP2; Green Fluoresecent Protein; GFP; Turbo GFP; eGFP; GFP tag|
|The GFP was isolated directly from the jellyfish Aequorea victoria.|
For Research Use Only. Not for use in diagnostic procedures.