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Thermo Scientific™ LanthaScreen™ TR-FRET ERR beta Coactivator Assay Kit, goat
The LanthaScreen™ TR-FRET ERR (estrogen related receptor) beta Coactivator Assay Kit provides a sensitive and robust method for highthroughput screening of potential ERR beta ligands as inverse agonists of ligand-dependent coactivator displacement.
Marca: Thermo Scientific™ PV4800
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Descripción
This kit contains Goat Tb-Anti-GST antibody; other kit components are the same as kit A15139:
The LanthaScreen™ TR-FRET ERR (estrogen related receptor) beta Coactivator Assay Kit provides a sensitive and robust method for highthroughput screening of potential ERR beta ligands as inverse agonists of ligand-dependent coactivator displacement. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a human recombinant ERR beta ligand-binding domain (ERR beta-LBD) that is tagged with glutathione-S-transferase (GST) in a homogeneous mix-and-read assay format.
To assay:
When running the LanthaScreen™ ERR beta TR-FRET Coactivator Assay, ERR beta-LBD is added to ligand test compounds followed by addition of a mixture of the fluorescein-coactivator peptide and Tb-labeled anti-GST antibody. After an incubation period at room temperature, the 520 nm/495 nm TR-FRET ratio is calculated and can be used to determine the EC50 from a dose response curve of the compound.
Based on the biology of the ERR beta-coactivator peptide interaction, this ligand EC50 is a composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and displace coactivator peptide (see Figure 1).
Especificaciones
| Tb (terbio) | |
| Fluorescente | |
| Proteína ERR beta-LBD (GST), un péptido coactivador de PGC-1a etiquetado con fluorescencia, anticuerpo anti-GST etiquetado con Tb y tampones | |
| Para uso exclusivo en investigación (RUO) | |
| El kit de ensayo del coactivador de ERR beta LanthaScreen™ TR-FRET contiene proteína ERR beta-LBD (GST), péptido coactivador de PGC1a marcado con fluorescencia, anticuerpo anti-GST marcado con Tb y tampones. Almacene los componentes como se indica en el protocolo de ensayo (-80 °C, -20 °C o +4 °C). |
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| Punto final | |
| NR3B2, ERR2, ESRRB | |
| Ensayo de interacción de cofactores, TR-FRET | |
| Kit de ensayo de coactivadores TR-FRET ERR Beta |
| Kit de ensayo de coactivadores de ERR beta TR-FRET LanthaScreen™, 800 ensayos de 20 μl, formato de placa de pocillos de 384 pocillos, tipo de colorante Tb (terbio), método de detección fluorescente, técnica TR-FRET, para farmacología y biofarmacología, identificación y validación de objetivos y secuencias líder | |
| Lector de microplacas | |
| Ensayos de 800 x 20 μL | |
| Placa de 384 pocillos | |
| Interacción de correguladores bioquímicos | |
| Hielo seco | |
| LanthaScreen™ | |
| ERR beta | |
| 800 x 20 μL Assays |
For Research Use Only. Not for use in diagnostic procedures.