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Invitrogen™ Human TNF-alpha Recombinant Protein

Código de producto. 10056073
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Cantidad:
20 μg
Tamaño de la unidad:
20 microgramos
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Código de producto. 10056073

Marca: Invitrogen™ RTNFAI

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Descripción

Recombinant Protein

The mature, soluble form of human TNFa was expressed in Pichia pastoris, KM71H. GenBank Accession # P01375, amino acids 77 - 233. For protein verification, the expressed protein was N-terminal sequenced and begins with the following 10 amino acids: VRSSSRTPSD. Purity is >95% by SDS-PAGE analyses. Activity: The biological activity of recombinant human TNFa was measured by its cytolytic effect on mouse L-929 cells in the presence of Actinomycin D. Use: For in vitro applications. A dose-response assay should be performed to determine the optimal concentration for specific experiments. Reconstitution and Dilution: Store lyophilized material at 4°C. Reconstitute in 1 mL of distilled or deionized water. Dilute in a buffer-containing carrier protein, aliquot, and store at or below -70°C, avoiding multiple freeze-thaw cycles. PBS with 1% BSA or a neutral media containing <10% FCS are suitable buffers.

TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.
TRUSTED_SUSTAINABILITY

Especificaciones

Número de acceso P01375
Para utilizar con (aplicación) Bioactivity
Formulación PBS with no preservative
ID de gen (Entrez) 7124
Peso molecular 17.4 kDa
Nombre Human TNF-alpha
Método de purificación Purified
Cantidad 20 μg
Fuente Yeast
Requisitos de almacenamiento 4°C
Estado normativo RUO
Alias de gen APC1 protein; Cachectin; C-domain 1; C-domain 2; cTNF; DADB-70P7.1; DIF; ICD1; ICD2; Intracellular domain 1; Intracellular domain 2; N-terminal fragment; NTF; RATTNF; Tnf; TNF alpha; TNF superfamily; TNF α; TNF, macrophage-derived; TNF, monocyte-derived; TNFA; TNF-a; TNFalpha; TNF-alpha; Tnfsf1a; TNFSF2; TNFα; TNLG1F; Tumor necrosis factor; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; tumor necrosis factor alpha (cachetin); tumor necrosis factor alpha precursor; tumor necrosis factor ligand 1F; tumor necrosis factor ligand superfamily member 2; Tumor necrosis factor, membrane form; Tumor necrosis factor, soluble form; tumor necrosis factor-alpha; tumor necrosis factor-alpha precursor; tumor-necrosis factor; tumour necrosis factor
Nombre común TNF alpha
Símbolo de gen Tnf
Actividad biológica Determined by the cytolytic effect on mouse L-929 cells in the presence of Actinomycin D.
Tipo de producto Protein
Conjugado Unconjugated
Especie Yeast
Recombinante Recombinant
Secuencia Human TNF-alpha, amino acids 77-233 (Accession # P01375)
Contenido y almacenamiento 4°C
Actividad Determined by the cytolytic effect on mouse L-929 cells in the presence of Actinomycin D.
Sistema de expresión P. pastoris
Formulario Lyophilized
Subtipo de proteína Recombinant
Grado de pureza o calidad >95% by SDS-PAGE
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